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Series GSE117156 Query DataSets for GSE117156
Status Public on Dec 07, 2018
Title Single cell dissection of plasma cell heterogeneity in symptomatic and asymptomatic myeloma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Multiple myeloma (MM), a plasma cell (PC) malignancy, is the second most common blood cancer. Despite extensive research, disease heterogeneity within and between patients is poorly characterized, hampering efforts for early diagnosis and improved treatments. Here, we apply single cell RNA-seq to study the heterogeneity of 40 individuals along the MM progression spectrum. We define malignant PC at single cell resolution, demonstrating high inter-patient variability that can be explained by expression of known MM drivers and additional putative factors. Within newly diagnosed patients, we identify extensive sub-clonal structures for 10/29 patients. In asymptomatic patients with early disease and in minimal residual disease post-treatment, we detect tumor PC for a subset of the patients, with the same drivers of active myeloma. Single cell analysis of rare circulating tumor cells (CTC) allows detection of malignant PC, which reflect the BM disease. Our work establishes scRNA-seq for dissecting blood malignancies and devising detailed molecular characterization of tumor cells in symptomatic and asymptomatic patients.
Overall design The study includes 29 newly diagnosed patients with plasma cell neoplasms and 11 control donors, for which bone marrow plasma cells were single cell sorted by FACS, and their mRNA sequenced. For 11 patients, targeted genomic DNA panel analysis for myeloma was performed.
Web link
Contributor(s) Ledergor G, Weiner A, Papaemmanuil E, Amit I
Citation(s) 30523328
Submission date Jul 16, 2018
Last update date Mar 26, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (135)
GSM3272359 AB2136
GSM3272364 AB2137
GSM3272366 AB2138
BioProject PRJNA481319
SRA SRP154380

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117156_RAW.tar 93.3 Mb (http)(custom) TAR (of TXT)
GSE117156_metadata.txt.gz 408.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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