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| Status |
Public on Oct 29, 2008 |
| Title |
TGF-beta-induced gene expression data and Smad2/3 binding sites of HaCaT keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. Here we analyzed the effect of identified Smad2/3 binding sites to transcription. We used expression microarrays to compare the Smad2/3 binding sites identified by ChIP-chip to TGF-beta-induced gene expressions. Keywords: time course
We also examined the effect of either ETS1/TFAP2A/SMAD2/SMAD3 siRNAs on TGF-beta-induced gene expression change.
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| |
|
| Overall design |
HaCaT cells were treated with TGF-beta3 for Smad2/3 ChIP-chip or RNA extraction and hybridization on Affymetrix microarrays.
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| |
|
| Contributor(s) |
Koinuma D, Tsutsumi S |
| Citation(s) |
18955504 |
| |
| Submission date |
Jun 09, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
|
| Organization name |
University of Tokyo
|
| Department |
Pathology
|
| Street address |
Hongo 7-3-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL7026 |
Human Promoter Array 1.0R - NCBI build_35 |
|
| Samples (20)
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|
| Relations |
| BioProject |
PRJNA106007 |
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