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Series GSE117055 Query DataSets for GSE117055
Status Public on Sep 09, 2018
Title Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Spiral ganglion (SG) neurons of the cochlea convey all auditory inputs to the brain, yet the cellular and molecular complexity necessary to decode the various acoustic features in the SG has remained unresolved. Using unbiased single-cell RNA sequencing, we identified four types of SG neurons, including three novel subclasses of type I neurons and the type II neurons, and provide a comprehensive genetic framework that define their potential synaptic communication patterns. The connectivity patterns of the three subclasses of type I neurons with inner hair cells and their electrophysiological profiles suggest that they represent the intensity-coding properties of auditory afferents. Moreover, neuron type specification is already established at birth, indicating a neuronal diversification process independent of neuronal activity. Thus, this work provides a transcriptional catalog of neuron types in the cochlea, which serves as a valuable resource for dissecting cell-type-specific functions of dedicated afferents in auditory perception and in hearing disorders.
 
Overall design Exploring heterogeneity of spiral ganglion neurons in both P3 and adult mice

Please note that the single cells were sorted into 384-well plates, and cells from each well were barcoded afterwards using Nextera index adapters during library preparation. Therefore the number of the well (A1-P24) represents each cell (A1-P24).
 
Contributor(s) Petitpré C, Wu H, Sharma A, Tokarska A, Fontanet P, Wang Y, Helmbacher F, Yackle K, Silberberg G, Hadjab S, Lallemend F
Citation(s) 30209249
Submission date Jul 12, 2018
Last update date May 15, 2019
Contact name Francois Lallemend
E-mail francois.lallemend@ki.se
Organization name Karolinska Institutet
Department Neuroscience
Street address TOMTEBODAVAGEN 16
City Stockholm
State/province Stockholm
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM3267886 P3 EphA4-GFP
GSM3267887 P3 PvCre;Tom
GSM3267888 P17 PvCre;Tom
Relations
BioProject PRJNA480914
SRA SRP159855

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117055_RAW.tar 16.1 Mb (http)(custom) TAR (of TAB)
Raw data not provided for this record
Processed data provided as supplementary file
Raw data are available in SRA

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