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| Status |
Public on Jul 12, 2021 |
| Title |
Visual experience-dependent expression of Fn14 is required for retinogeniculate refinement (stimulus single nucleus RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sensory experience influences the establishment of neural connectivity through molecular mechanisms that remain unclear. Here, we employ single-nucleus RNA-sequencing to investigate the contribution of sensory-driven gene expression to synaptic refinement in the dorsal lateral geniculate nucleus of the thalamus, a region of the brain that processes visual information. We find that visual experience induces the expression of the cytokine receptor Fn14 in excitatory thalamocortical neurons. By combining electrophysiological and structural techniques, we show that Fn14 is dispensable for early phases of refinement mediated by spontaneous activity, but that Fn14 is essential for refinement during a later, experience-dependent period of development. Refinement deficits in mice lacking Fn14 are associated with functionally weaker and structurally smaller retinogeniculate inputs, indicating that Fn14 mediates both functional and anatomical rearrangements in response to sensory experience. These findings identify Fn14 as a molecular link between sensory-driven gene expression and vision-sensitive refinement in the brain.
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| Overall design |
Single-nucleus RNA-sequencing was performed on microdissected dLGN tissue from C57Bl/6J WT mice (male and female) following dark-rearing and re-exposure to light for 0, 1, or 8 hours. Differential gene expression analysis was performed to compare 1 or 8 hours of stimulation with 0 hours.
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| Contributor(s) |
Cheadle L, Tzeng CP, Kalish BT, Harmin DA, Nagy MA, Greenberg ME |
| Citation missing |
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| Submission date |
Jul 12, 2018 |
| Last update date |
Jul 13, 2021 |
| Contact name |
Lucas Martin Cheadle |
| E-mail(s) |
lucas_cheadle@hms.harvard.edu
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| Organization name |
Harvard Medical School
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| Department |
Neurobiology
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| Lab |
Greenberg
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| Street address |
220 Longwood Ave; Goldenson Bldg 413
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
| GSM3267399 |
Mouse LGN snRNAseq 0 -8 hour stim rep 1-4 (12 samples) |
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| This SubSeries is part of SuperSeries: |
| GSE117024 |
Visual experience-dependent expression of Fn14 is required for retinogeniculate refinement |
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| Relations |
| BioProject |
PRJNA480865 |
| SRA |
SRP153193 |
