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| Status |
Public on Jul 01, 2021 |
| Title |
Temporal controls over interareal cortical projection neuron fate diversity (retrobeads) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interconnectivity between neocortical areas is critical for sensory integration and sensorimotor transformations. These functions are mediated by heterogeneous interareal cortical projection neurons (ICPN), which send axon branches to distinct cortical areas as well as to subcortical targets. Although ICPN are anatomically diverse, they are molecularly homogeneous and how the diversity of their anatomical and functional features emerge during development remains largely unknown. Here, we address this question by linking connectome and transcriptome in developing single ICPN of the mouse neocortex using a combination of MAPseq mapping (to identify single-neuron axonal projections) and single-cell RNA sequencing (to identify corresponding gene expression). Focusing on neurons of the primary somatosensory cortex (S1), we reveal a protracted unfolding of the molecular and functional differentiation of motor cortex-projecting (M) compared to secondary somatosensory cortex-projecting (S2) ICPN. We identify SOX11 as a temporally differentially expressed transcription factor in M vs. S2 ICPN. Postnatal manipulation of SOX11 expression level in S1 impaired sensorimotor connectivity and selectively disrupted exploratory behavior in freely moving mice. Together, our results reveal that within a single cortical area, different subtypes of ICPN have distinct postnatal molecular differentiation paces, which is then reflected in distinct circuit connectivities and functions. Dynamic differences in expression levels of largely generic set of genes, rather than fundamental differences in the identity of developmental genetic programs, may thus account for emergence of intra-type diversity in cortical neurons.
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| Overall design |
This record contains single cell transcriptomics data from one Fluidigm integrated fluidic circuits. Retrobeads are injected in cerebral cortex M1 and S2 of P9 mouse. Primary somatosensory cortex is dissected and FACS-sorted to select for cells with retrobeads that project either to M1 or S2. The transcriptome of these S1 cells is sequenced with using Fluidigm integrated fluidic circuits on C1 system. The goal of this experiment is to determine genes patterns of cells projecting to either M1 or S2.
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| Contributor(s) |
Esther K, Tomasello U, Prados J, Kebschull J, Contestabile A, Galiñanes G, Fièvre S, Huber D, Dayer A, Bellone C, Santinha A, Platt R, Denis J |
| Citation(s) |
34754107 |
| Submission date |
Jul 11, 2018 |
| Last update date |
Nov 19, 2021 |
| Contact name |
Julien Prados |
| E-mail(s) |
julien.prados@unige.ch
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| Phone |
+41 22 37 95 396
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| Organization name |
University of Geneva
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| Department |
SCMU
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| Lab |
Bioinformatics Support Platform
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| Street address |
Rue Michel Servet 1
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| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (316)
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| Relations |
| BioProject |
PRJNA480667 |
| SRA |
SRP153059 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116944_rbeads_cells_metadata.tsv.gz |
15.4 Kb |
(ftp)(http) |
TSV |
| GSE116944_sc_retrobeads.tsv.gz |
2.5 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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