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| Status |
Public on Jul 26, 2019 |
| Title |
Whole-transcriptome microarray analysis of grapevine-Botrytis cinerea interaction during latent infection of berries (“noble rot”) |
| Organism |
Vitis vinifera |
| Experiment type |
Expression profiling by array
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| Summary |
Purpose: Microarray technologies provide a unique opportunity to deeply investigate the molecular mechanisms involved in plant-pathogen interaction. Botrytis cinerea, is the agent of grapevine grey mould, but in yet uncharacterized environmental conditions, a latent infection can occur determining favourable metabolic and physico-chemical berry modifications which possibly contribute to the typical aromas of “passito” wines (“noble rot”). The present project aims at the identification of the grapevine responses to B. cinerea during fungal colonization in the latent form, in comparison with control berries. Methods: A total of 150 untreated berries were sampled as time 0 of the experiment. Moreover, 300 healthy berries have been artificially inoculated one by one with B. cinerea by injecting conidia under berry skin, in controlled conditions, reproducing an early stage (T1) and a late stage (T2) of noble rot pourri plein. Control samples (300 berries) have been inoculated with water and sampled at the same time of infected berries. The microarray experiments on T0 and healthy or infected samples in biological triplicate resulted in 15 samples to be analyzed (Agilent-048771 4x44K Grape all custom microarray chip; Agilent Technologies, Santa Clara, CA, USA). Conclusions: This work identified important molecular mechanisms involved in Botrytis cinerea colonization of grapevine berries during the noble rot infection.
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| Overall design |
mRNA profiles of Muller-Thurgau berries infected with Botrytis cinerea (noble rot) at T1 and T2 stage of pourri plein were either compared to mRNA profiles of uninfected Garganega berries (control berries) or to those T0 samples. All mRNA profiles were generated from three biological replicates.
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| Contributor(s) |
Lovato A, Colombo T, Tornielli GB, Polverari A, Vandelle E, Zenoni S |
| Citation(s) |
31304217 |
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| Submission date |
Jul 06, 2018 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Teresa Colombo |
| Organization name |
Consiglio Nazionale delle Ricerche (CNR)
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| Department |
Institute of Molecular Biology and Pathology (IBPM)
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| Street address |
p.le A. Moro 7
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| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL22427 |
Agilent-048771 4x44K Grape all [Probe Name version] |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE116741 |
Whole-transcriptome analysis of grapevine-Botrytis cinerea interaction during latent infection of berries |
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| Relations |
| BioProject |
PRJNA480023 |
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