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Series GSE11670 Query DataSets for GSE11670
Status Public on May 20, 2009
Title Transcriptional profiling of ICL670 treated K562 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Iron plays a central role in the regulation of many cellular functions. Dysregulation of its metabolism leads an iron overload situation and iron depletion leads to an inhibition of cell proliferation. Recent reports demonstrated that ICL670 (Novartis) acts as a potent NF-kappa-B inhibitor and improves hematological data in a subset of MDS patients (Cilloni et al, Haematologica, s1: 238, 2007). However, the precise mechanism of anti-cancer effect of ICL670 is still uncertain.
To evaluate the effect of ICL670, and to identify the moleclular pathways responsible for the observed reduced transfusion requirement during chelation therapy, we performed gene expression profiling to focus on the pathway involved in the anti-cancer effect of ICL670.
Overall design A human myeloid leukemia cell line, K562, was incubated with or without ICL670 for 16hrs.Gene expression profiling was done using an Affymerix GeneChip (U133 Plus 2.0). Statistical analysis was done by GeneSifter vizXlab, Seattle, WA).
Contributor(s) Ohyashiki JH
Citation(s) 19298223
Submission date Jun 04, 2008
Last update date Mar 25, 2019
Contact name Junko H Ohyashiki
Phone +81-3-3342-6111(ext.5999)
Fax +81-3-5381-6651
Organization name Tokyo Medical University
Department Intractable Immune System Diseases Research Center
Street address 6-7-1, Nishishinjuku, Shinjuku-ku
City Tokyo
ZIP/Postal code 160-0023
Country Japan
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM296608 K562_ICL670_untreated_dup1
GSM296609 K562_ICL670_untreated_dup2
GSM296612 K562_ICL670_10uM_dup1
BioProject PRJNA106069

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11670_RAW.tar 48.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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