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Series GSE11618 Query DataSets for GSE11618
Status Public on Dec 01, 2008
Title Stable XIAP knockdown in HCT116 colon cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary X-linked inhibitor of apoptosis (XIAP) is the most potent endogenous caspase inhibitor preventing cell death via caspase-9, -7 and -3 (initiator and executioner caspase pathways). Using short hairpin RNA (shRNA) against XIAP, stably expressed in a parent HCT116 human colon cancer cell line, a series of clones have been developed. XIAP mRNA levels were established by RT-PCR, the four X (XIAP knockdown) clonal cell lines show 82-93% reduction in XIAP mRNA when compared to the four L (luciferase control) cell lines. Immunoblot analysis showed a 67-89% reduction in XIAP protein in X cell lines compared to L. RNA was analysed by microarray and XIAP knockdown was confirmed in 7 probe sets, there was no significant compensation of other IAP family members. XIAP knockdown induced a 2-fold increase in the basal level of apoptosis without modification of caspase 3/7 activity. Finally, XIAP knockdown sensitises cells to radiotherapy by 20%, to recombinant TRAIL by a 3-fold factor, and to paclitaxel and docetaxel by >2 fold factor. Future work should focus on targeted agents such as rhTRAIL in combination with strategies to down regulate XIAP. XIAP antisense is now in clinical development in oncology.
Overall design Human HCT116 colon cancer cell lines were transfected with either XIAP (X) or luciferase (L) shRNA. The expression profiles of both were compared at early (p4) and late (p8) passage.
Contributor(s) Connolly KC, Mitter R, Muir M, Jodwell DI, Guichard SM
Citation(s) 19034449
Submission date May 30, 2008
Last update date Mar 25, 2019
Contact name Kathryn Clare Connolly
Organization name University of Edinburgh
Department Cancer Research Centre
Lab Pharmacology and Drug Development Group
Street address Crewe Road South
City Edinburgh
ZIP/Postal code EH4 2XR
Country United Kingdom
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (18)
GSM294802 L_p4_rep1
GSM294803 L_p4_rep2
GSM294804 L_p4_rep3
BioProject PRJNA106125

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Supplementary file Size Download File type/resource
GSE11618_RAW.tar 86.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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