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| Status |
Public on Jun 26, 2018 |
| Title |
DNA methylation oscillations at CpG poor enhancers in primed embryonic stem cells [amplicon-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Mouse embryonic stem cells (ESCs) primed for differentiation display dynamic heterogeneity characterised by stochastic switching between transcriptional states, and recent advances have highlighted the heterogeneous and dynamic nature of DNA methylation in these. Using single cell sequencing we report global oscillations in ESC DNA methylation that affect particularly CpG poor regions including distal enhancers. These oscillations are dependent on DNA methylation turnover by Dnmt3 and Tet enzymes and influence the probability of transcriptional state switching. Our observations suggest that regulated DNA methylation heterogeneity may contribute to lineage priming in cells poised for differentiation.
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| Overall design |
2i ES cells transferred into serum media and collected at 48 time points. Control cells were 2i ES cells collected at the same time points (i.e. without changing to serum media)
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| Contributor(s) |
Rulands S, Lee HJ, Clark SJ, Angermueller C, Smallwood SA, Krueger F, Kelsey G, Stegle O, Simons BD, Reik W |
| Citation(s) |
30031774 |
| |
| Submission date |
Jun 22, 2018 |
| Last update date |
Jan 27, 2019 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
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| Organization name |
Altos Labs
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| Department |
Bioinformatics
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| Street address |
Granta Park
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| City |
Cambridge |
| ZIP/Postal code |
CB21 6GP |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (96)
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| This SubSeries is part of SuperSeries: |
| GSE75975 |
Genome-scale DNA methylation oscillations in pluripotent cells |
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| Relations |
| BioProject |
PRJNA477530 |
| SRA |
SRP151135 |
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