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| Status |
Public on Jul 16, 2018 |
| Title |
Osterix functions downstream of anti-Müllerian hormone signaling to regulate Müllerian duct regression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to identify potential AMH-induced genes and regulatory networks controlling regression by RNA-Seq transcriptome analysis of differences in Müllerian Duct mesenchyme between males (AMH signaling on) and females (AMH signaling off) in purified fetal Müllerian Duct mesenchymal cells. This analysis found 82 genes up-regulated in males during MD regression and identified Osterix (Osx)/Sp7, a key transcriptional regulator of osteoblast differentiation and bone formation, as a novel downstream effector of AMH signaling during MD regression.
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| |
|
| Overall design |
Müllerian Duct mesenchymal cells mRNA profiles from 2-7 embryonic day 14.5 embryos were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000.
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| |
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| Contributor(s) |
Mullen RD, Behringer RR |
| Citation(s) |
30061417 |
| |
| Submission date |
Jun 22, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Rachel Diane Mullen |
| Organization name |
MD Anderson Cancer Center
|
| Department |
Genetics
|
| Lab |
Richard Behringer
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
| GSM3211518 |
Amhr2Cre/+, R26R YFP/+ female biological rep1 |
| GSM3211519 |
Amhr2Cre/+, R26R YFP/+ male biological rep1 |
| GSM3211520 |
Amhr2Cre/+, R26R YFP/+ female biological rep2 |
| GSM3211521 |
Amhr2Cre/+, R26R YFP/+ male biological rep2 |
| GSM3211522 |
Amhr2Cre/+, R26R YFP/+ male biological rep3 |
| GSM3211523 |
Amhr2Cre/+, R26R YFP/+ female biological rep3 |
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| Relations |
| BioProject |
PRJNA477505 |
| SRA |
SRP151113 |
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