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| Status |
Public on Apr 26, 2019 |
| Title |
Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence |
| Organism |
Cryptococcus neoformans H99 |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Lysine acetylation is critical in regulating important biological processes in many organisms, yet little is known about acetylome evolution and its contribution to phenotypic diversity. Here, we compare the acetylomes of baker’s yeast and the three deadliest human fungal pathogens, Cryptococcus neoformans, Candida albicans, and Aspergillus fumigatus. Using mass spectrometry enriched for acetylated peptides together with public data from Saccharomyces cerevisiae, we show that fungal acetylomes are characterized by dramatic evolutionary dynamics and limited conservation in core biological processes. Notably, the levels of protein acetylation in pathogenic fungi correlate with their pathogenicity. Using gene knockouts and pathogenity assays in mice, we identify deacetylases with critical roles in virulence and protein translation elongation. Finally, through mutational analysis of deactylation motifs we find evidence of positive selection at specific acetylation motifs in fungal pathogens. These results shed new light on the pathogenicity regulation mechanisms underlying the evolution of fungal acetylomes.
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| Overall design |
The paired-end RNA-seq and ChIP-seq of Cryptococcus neoformans H99s were performed using three biological replicates
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| Contributor(s) |
Ding C, Li Y, Li H, Sui M, Li M, Wang J, Meng Y, Sun T, Liang Q, Suo C, Gao X, Li C, Li Z, Du W, Zhang B, Sai S, Zhang Z, Ye J, Wang H, Yue S, Li J, Zhong M, Chen C, Qi S, Lu L, Li D |
| Citation(s) |
31069264 |
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| Submission date |
Jun 20, 2018 |
| Last update date |
May 15, 2019 |
| Contact name |
Li Hailong |
| E-mail(s) |
lihailong1166@gmail.com
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| Organization name |
Northeastern University
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| Street address |
NO. 3-11, Wenhua Road, Heping District
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| City |
ShenYang |
| State/province |
Liaoning |
| ZIP/Postal code |
110001 |
| Country |
China |
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| Platforms (2) |
| GPL23176 |
Illumina HiSeq 2500 (Cryptococcus neoformans var. grubii H99) |
| GPL24689 |
Illumina NextSeq 500 (Cryptococcus neoformans var. grubii H99) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA476875 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116040_RAW.tar |
47.8 Mb |
(http)(custom) |
TAR (of TXT, WIG) |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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