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Series GSE115982 Query DataSets for GSE115982
Status Public on Sep 04, 2018
Title Single cell RNA sequencing of cultured CCR10+ and CCR10- lung airway epithelial cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mechanically dissociated IPF lung explant derived cellular suspensions were cultured in 50% senescent lung fibroblast conditioned medium + 50% fibroblast complete medium (DMEM + 15% FBS + antiboitics) and 10 µM of Y27632. After approximately 2 weeks, cells were passaged and cultured overnight in Pneumacult Ex Plus medium (STEMCELL technologies).
 
Overall design Explanted IPF lung tissues were mechanically minced to release loosely adherent and non-adherent cells. Cells were purified by passing the dissociated suspensions through a sterile 100 µm filter and then cultured in CRC medium: 50% senescent lung fibroblast conditioned medium + 50% fibroblast complete medium (DMEM + 15% FBS + antiboitics) and 10 µM of Y27632. Medium was changed every 3 days for approximately two weeks. After two weeks, cells were dissociated using Accutase solution and replated in PnemaCult Ex Plus medium (STEMCELL technologies) containing 10 µM Y27632 overnight. The next morning epithelial cells were dissociated using Accutase solution, stained with anti-EpCAM and -CCR10 antibodies and EpCAM+ CCR10+ and EpCAM+ CCR10- epithelial cells were FACS sorted using a BD Aria III sorter. Single sorted cells were then caputred and sequenced using 10x genomics platform.
 
Citation(s) 30135312
Submission date Jun 19, 2018
Last update date Oct 11, 2021
Contact name AGCT Core
Organization name Cedars Sinai Medical Center
Department BMS
Street address 8687 Melrose Ave, PDC B230
City West Hollywood
State/province CA
ZIP/Postal code 90069
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM3204304 PP: EpCAM+ CCR10+
GSM3204305 PN: EpCAM+ CCR10-
Relations
BioProject PRJNA476693
SRA SRP151495

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Supplementary file Size Download File type/resource
GSE115982_RAW.tar 360.6 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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