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| Status |
Public on Jun 19, 2018 |
| Title |
p53 binding and chromatin dynamics after etoposide treatment of primary lung fibroblast |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the chromatin modification dynamics at p53 binding sites afte 6 hours of treatment of etoposide (100 micromolar final) in IMR90 human lung fibroblasts using ChIP-seq , ATAC-seq, and polyA+ RNA-seq analyses. We assessed the genomewide occupancy of histone H3, H3K4me3, H3K4me2, H3K4me1, H3K27ac, H4K16ac, RNA polymerase II, p53, and transposase-accessible chromatin (ATAC-seq) in response to etoposide treatment.
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| |
|
| Overall design |
ChIP-seq, RNA-seq, and ATAC-seq for histone modifications, p53, and RNA polymerase II after etoposide treatment in IMR90 fetal lung fibroblasts
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| |
|
| Contributor(s) |
Sammons M |
| Citation(s) |
30966857 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R15 GM128049 |
Molecular mechanisms regulating the establishment of cis-regulatory elements by the transcription factor p63 |
UNIVERSITY AT ALBANY, SUNY |
Morgan Andrew Sammons |
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| |
| Submission date |
Jun 18, 2018 |
| Last update date |
Sep 13, 2019 |
| Contact name |
Morgan A Sammons |
| E-mail(s) |
masammons@albany.edu
|
| Organization name |
University at Albany - SUNY
|
| Department |
Biological Sciences
|
| Lab |
Sammons
|
| Street address |
1400 Washington Ave, Life Sciences 2075
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA476558 |
| SRA |
SRP150762 |
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