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Series GSE115607 Query DataSets for GSE115607
Status Public on Jun 12, 2018
Title ChIP-Seq analysis of estrogen deprived MCF7 cells treated with H3B-5942 and standards of care compounds
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The goal of this experiment was to interrogate the potential transcriptional impact of H3B-5942 by investigating its influence on genome-wide DNA-binding modes of ERa in MCF7 cells using chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-Seq). Affects of H3B-5942 on chromatin recruitment were compared to results from treatment with E2, 4-OHT, and fulvestrant.
Overall design A total of 5 treatments were analyzed. These included H3B-5942, E2, reference compounds 4-OHT and fulvestrant, and DMSO as control. Material from two plates per treatment were pooled and then analyzed.
Contributor(s) Furman C, Wu ZJ
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Submission date Jun 11, 2018
Last update date Mar 26, 2019
Contact name Zhenhua Wu
Phone 6179592279
Organization name H3 Biomedicine
Street address 300 Technology Square floor 5
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (11)
GSM3184871 ERalpha s1 DMSO ChIPSeq
GSM3184872 ERalpha s2 6545 ChIPSeq
GSM3184873 ERalpha s3 s6545 ChIPSeq
This SubSeries is part of SuperSeries:
GSE115611 Discovery of Selective Estrogen Receptor Covalent Antagonists (SERCAs) for the treatment of ERa(WT) and ERa(MUT) breast cancer.
BioProject PRJNA475564
SRA SRP150236

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115607_RAW.tar 2.1 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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