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| Status |
Public on Jun 12, 2018 |
| Title |
ChIP-Seq analysis of estrogen deprived MCF7 cells treated with H3B-5942 and standards of care compounds |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The goal of this experiment was to interrogate the potential transcriptional impact of H3B-5942 by investigating its influence on genome-wide DNA-binding modes of ERa in MCF7 cells using chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-Seq). Affects of H3B-5942 on chromatin recruitment were compared to results from treatment with E2, 4-OHT, and fulvestrant.
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| Overall design |
A total of 5 treatments were analyzed. These included H3B-5942, E2, reference compounds 4-OHT and fulvestrant, and DMSO as control. Material from two plates per treatment were pooled and then analyzed.
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| Contributor(s) |
Furman C, Wu ZJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 11, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Zhenhua Wu |
| E-mail(s) |
zhenhuawu75@gmail.com
|
| Phone |
6179592279
|
| Organization name |
H3 Biomedicine
|
| Street address |
300 Technology Square floor 5
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE115611 |
Discovery of Selective Estrogen Receptor Covalent Antagonists (SERCAs) for the treatment of ERa(WT) and ERa(MUT) breast cancer. |
|
| Relations |
| BioProject |
PRJNA475564 |
| SRA |
SRP150236 |
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