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Series GSE11558 Query DataSets for GSE11558
Status Public on Sep 01, 2008
Title transcript profiling of the adaptive response to decreases in oxygen concentration in the roots of Arabidopsis plants
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary - Background and Aims: Oxygen can fall to low concentrations within plant tissues, either because of environmental factors that decrease the external oxygen concentration or because the movement of oxygen through the plant tissues cannot keep pace with the rate of oxygen consumption. Recent studies document that plants can decrease their oxygen consumption in response to relative small changes in oxygen concentrations to avoid internal anoxia. The molecular mechanisms underlying this response have not been identified yet. The aim of this study was to use transcript and metabolite profiling to investigate the genomic response of Arabidopsis roots to a mild decrease in oxygen concentrations.
- Methods: Arabidopsis seedlings were grown on vertical agar plates at 21, 8, 4 and 1% (v/v) external oxygen for 0.5, 2 and 48h. Roots were analyzed for changes in transcript levels using Affymetrix whole genome DNA microarrays, and for changes in metabolite levels using routine GC-MS based metabolite profiling. Root extension rates were monitored in parallel to investigate adaptive changes in growth.
- Key results: Results show that root growth was inhibited and transcript and metabolite profiles were significantly altered in response to a moderate decrease in oxygen concentrations. Low oxygen leads to a preferential up-regulation of genes that might be important to trigger adaptive responses in the plant. A small but highly specific set of genes is induced very early in response to a moderate decrease in oxygen concentrations. Genes that were down-regulated mainly encoded proteins involved in energy-consuming processes. In line with this, root extension growth was significantly decreased which will ultimately save ATP and decrease oxygen consumption. This was accompanied by a differential regulation of metabolite levels at short and long term incubation at low oxygen.
- Conclusions: Results show that there are adaptive changes in root extension involving large-scale reprogramming of gene expression and metabolism when oxygen concentration is decreased in a very narrow range.
 
Overall design Arabidopsis seedlings were grown on vertical agar plates and treated with various oxygen concentrations (1%, 4%, 8%, and 21% as a control), 350ppm CO2 and N2 (rest) for different time periods (0.5 hours, 2 hours and 2 days. At the end of the experiment, the roots of the seedlings were immediately frozen in liquid nitrogen and used for gene expression analysis.
 
Contributor(s) van Dongen JT, Froehlich A, Geigenberger P
Citation(s) 18660497
Submission date May 26, 2008
Last update date Aug 28, 2018
Contact name Joost T. van Dongen
E-mail(s) dongen@mpimp-golm.mpg.de
Phone +49 (0)331 5678353
URL http://www.mpimp-golm.mpg.de
Organization name Max Planck Institute of Molecular Plant Physiology
Street address Am Muehlenberg 1
City Potsdam
ZIP/Postal code D-14476
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (28)
GSM290753 root - 01% oxygen - 2h - E
GSM290755 root - 01% oxygen - 2h - F
GSM290756 root - 01% oxygen - 30min - D
Relations
Affiliated with GSE69995
BioProject PRJNA106291

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11558_RAW.tar 269.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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