|
| Status |
Public on Jul 22, 2009 |
| Title |
Downregulation of cinnamoyl-coenzyme A reductase in maize (affy_ccr_maize) |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
|
| Summary |
affy_ccr_maize - affy_ccr_maize - Cinnamoyl-CoA reductase (CCR) catalyzes a key step in monolignol biosynthesis. We show that downregulation of CCR in maize was associated with lower lignin content and a strong decrease in H units. Concomitantly, these cell wall modifications were associated with higher digestibility. On another hand, immunocytochemistry indicated a modification of lignification pattern and cellulose content. Transcript profiling was used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. -2 wild type and 2 CCR mutants were compared. Plants were grown in greenhouse condition and harvested at 7-8 leaf stages.
Keywords: gene knock in (transgenic)
|
| |
|
| Overall design |
4 arrays - maize
|
| Web link |
http://urgv.evry.inra.fr/CATdb
|
| |
|
| Contributor(s) |
Balzergue S, Goffner D, Pichon M, Martin-Magniette ML |
| Citation(s) |
21493812 |
| Submission date |
May 22, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
|
| Samples (4)
|
| GSM290553 |
WS1 with array type maize from Affymetrix |
| GSM290554 |
WS2 with array type maize from Affymetrix |
| GSM290555 |
CCR mutant M1 with array type maize from Affymetrix |
| GSM290556 |
CCR mutant M2 with array type maize from Affymetrix |
|
| Relations |
| BioProject |
PRJNA106251 |
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