|
| Status |
Public on Jun 11, 2018 |
| Title |
Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The human pluripotent stem cells cultured on Low-adhesion substrates formed two morphologically different subtypes (Monolayer and Domed). The dome-like cells showed higher proliferation capacity and KLF4/5 expression than the monolayer cells. A serum response factor based regulatory double loop was proposed to explain how cell-matrix adhesion mediates the interaction between cell morphology and pluripotency genes
|
| |
|
| Overall design |
Compare the two type cells with RNA-seq to investigate the different expression
|
| |
|
| Contributor(s) |
Dang Y, Yu L, Hou Y, Liu L |
| Citation(s) |
30008324 |
| Submission date |
Jun 03, 2018 |
| Last update date |
May 21, 2019 |
| Contact name |
Leqian Yu |
| E-mail(s) |
ylq.urakuken@gmail.com
|
| Organization name |
Kyoto university
|
| Street address |
205 Room, International Science Innovation Building
|
| City |
Kyoto |
| ZIP/Postal code |
6068501 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA474380 |
| SRA |
SRP149631 |
Less...
More...