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Status |
Public on Jun 02, 2018 |
Title |
Oncogenic KRAS(G12V) and BRAF(V600E) in intestinal organoids |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Goals of the study was to compare transcripional and phenotypic response of mouse intestinal organoid cultures to the KRAS(G12V) or BRAF(V600E)oncogenes.
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Overall design |
Two biological replicates of organoids with transgenic luc-tdTomato, KRAS(G12V)-tdTomato, BRAF(V600E)-tdTomato were analysed by RNA-Seq By comparing 7-10 x 10E7 50bp paired end reads per library we identify transcriptional alterations in the intestinal epithelium following expression of each oncogene
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Contributor(s) |
Riemer P, Morkel M |
Citation(s) |
31266962 |
Submission date |
Jun 01, 2018 |
Last update date |
Jul 16, 2019 |
Contact name |
Markus Morkel |
E-mail(s) |
markus.morkel@charite.de
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Organization name |
Charité - Universitätsmedizin Berlin
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Department |
Dept. of Pathology
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Lab |
Laboratory of Molecular Tumour Pathology
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Street address |
Charitéplatz 1
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City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA474218 |
SRA |
SRP149567 |