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| Status |
Public on Dec 02, 2018 |
| Title |
RNAseq analysis of whole islets from pre-weaning wild type and Akita mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study aims to determine the effects of proinsulin misfolding/ER stress on β-cell dynamics and function in Akita mice. The RNAseq experiment offers the opportunity to investigate the impact of proinsulin misfolding/ ER stress on the genetic signature of islets isolated during the neonatal period in the Akita mice preceding the development of hyperglycemia.
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| Overall design |
P19-21 Akita and age-matched control mice (n=3 in each group, each sample is pooling of islets from 3 mice) were isolated and processed for RNA exctraction. RNA sequencing libraries were then constructed from 120 ng of total RNA.
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| Contributor(s) |
Riahi Y, Leibowitz G |
| Citation(s) |
30412050 |
| |
| Submission date |
May 25, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Yael Riahi |
| E-mail(s) |
yael.riahi@mail.huji.ac.il
|
| Organization name |
Hadassah Medical Center
|
| Street address |
Ein Kerem
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA473141 |
| SRA |
SRP148980 |
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