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Series GSE114895 Query DataSets for GSE114895
Status Public on Jun 03, 2018
Title Thymic tuft cell single-cell mRNA analysis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of the study was to sequence mRNA from tuft cells (identified as CD11c-;CD45-;EpCAM+;IL-25+ using Flare25 reporter mice) in C57BL/6 and Aire-/- mice. The data were used to investigate thymic tuft cell heterogeneity with and without AIRE protein expression.
Overall design Single cells prepared from pooled, digested, thymi were stained for flow cytometry and individual cells were sorted into wells of a 96 well plate containg 5 ul of lysis buffer. As controls, several wells were left blank (0 cells) or 100 cells were sorted.
Contributor(s) Miller CN, Wells KL, Rajpurkar AR, Steinmetz LM, Anderson MS
Citation(s) 30022164
Submission date May 24, 2018
Last update date Mar 21, 2019
Contact name Aparna Rajiv Rajpurkar
Organization name Stanford University
Department Genetics
Lab Steinmetz
Street address 279 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (319)
GSM3153839 WT [June29_KW.10.featurecounts]
GSM3153840 WT [June29_KW.11.featurecounts]
GSM3153841 WT [June29_KW.12.featurecounts]
BioProject PRJNA472990
SRA SRP148902

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114895_POR_all_data.rda.gz 14.6 Mb (ftp)(http) RDA
GSE114895_POR_all_data.txt.gz 32.2 Mb (ftp)(http) TXT
GSE114895_aireko_counts.txt.gz 1.2 Mb (ftp)(http) TXT
GSE114895_all_wt_counts.txt.gz 2.2 Mb (ftp)(http) TXT
GSE114895_samples_all.txt.gz 880 b (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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