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Series GSE114791 Query DataSets for GSE114791
Status Public on Jul 10, 2019
Title Kinome profiling of Non-Hodgkin’s lymphoma reveals Tyro3 is important in primary effusion lymphoma survival
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Non-Hodgkin’s lymphomas (NHL) make up the majority of lymphoma diagnoses and represent a very diverse set of malignancies. We sought to identify kinases uniquely upregulated in different NHL subtypes. Using Multiplexed Inhibitor Bead-mass spectrometry (MIB/MS), we found Tyro3 was uniquely upregulated and important for cell survival in primary effusion lymphoma (PEL). We developed an inhibitor against Tyro3 named UNC3810A, which inhibited cell growth in PEL but not in other NHL subtypes. UNC3810A also significantly inhibited tumor progression in a PEL xenograft mouse model compared to the vehicle treated animals. Our data suggests Tyro3 may be a therapeutic target for PEL.
 
Overall design RNASeq and analysis of 24 NHL cell lines. RNA was harvested from cell lines using the RNeasy Plus Mini Kit according to manufacturer’s instructions. mRNA-Seq libraries were generated using the Stranded mRNA-Seq kit and multiplexed with Illumina TruSeq adapters. Samples were run on two 75-cycle single-end sequencing runs with an Illumina NextSeq-500.
 
Contributor(s) Wong JP, Stuhlmiller TJ, Giffin LC, Lin C, Park SI, Johnson GL, Damania B
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Submission date May 22, 2018
Last update date Jul 10, 2019
Contact name Jason Paul Wong
E-mail(s) wongjp@email.unc.edu
Organization name University of North Carolina at Chapel Hill
Department Microbiology and Immunology
Street address Lineberger Comprehensive Cancer Center, 450 West Dr.
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM3150236 BC1
GSM3150237 BC3
GSM3150238 BCBL1
Relations
BioProject PRJNA472630
SRA SRP148762

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114791_RNAseq_Lymphoma_Cell_Lines.xlsx 5.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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