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Series GSE114763 Query DataSets for GSE114763
Status Public on May 23, 2018
Title Human Muscle Posses Epigenetic Memory
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary It is unknown if adult human skeletal muscle has an epigenetic memory of earlier encounters with growth. We report, for the first time in humans, genome-wide DNA methylation (850,000CpGs) and gene expression analysis after muscle hypertrophy (loading), return of muscle mass to baseline (unloading), followed by later hypertrophy (reloading). We discovered increased frequency of hypomethylation across the genome after reloading (18,816 CpGs) versus earlier loading (9,153 CpG sites). We also identified AXIN1, GRIK2, CAMK4, TRAF1 as hypomethylated genes with enhanced expression after loading that maintained their hypomethylated status even during unloading where muscle mass returned to control levels, indicating a memory of these genes methylation signatures following earlier hypertrophy. Further, UBR5, RPL35a, HEG1, PLA2G16, SETD3 displayed hypomethylation and enhanced gene expression following loading, and demonstrated the largest increases in hypomethylation, gene expression and muscle mass after later reloading, indicating an epigenetic memory in these genes. Finally, genes; GRIK2, TRAF1, BICC1, STAG1 were epigenetically sensitive genes demonstrating hypomethylation after a single bout of resistance exercise that was maintained 22 weeks later with the largest increase in gene expression and muscle mass after reloading. Overall, we identify an important epigenetic role for a number of largely unstudied genes in muscle hypertrophy/ memory.
Overall design Using a within-subjects design, skeletal muscle samples from the vastus lateralis were analsyed at baseline ((control; N=9; subject 1 has a duplicate(rep2) all other subjects are in single (rep1)), immediately (30-mins) following one single bout of acute resistance exercise (N=8; where this session was the first session of the subsequent 7-week training intervention) after 7 weeks of chronic resistance exercise loading (N=8), following a further 7 weeks of unloading (N=8) and finally, following 7 weeks of chronic resistance exercise reloading (N=7). All samples are run in single unless otherwise stated.
Contributor(s) Seaborne RA, Sharples AP
Citation(s) 29382913, 30375987
Submission date May 22, 2018
Last update date Jan 20, 2019
Contact name Robert Arthur Seaborne
Organization name Liverpool John Moores University
Department SCAMP
Street address Byrom Street
City Liverpool
ZIP/Postal code L3 3AF
Country United Kingdom
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (40)
GSM3149860 SkM_Epi_Mem_1: Muscle_Baseline_Participant_001_Rep1
GSM3149861 SkM_Epi_Mem_2: Muscle_Acute_Participant_001_Rep1
GSM3149862 SkM_Epi_Mem_3: Muscle_7wk_Loading_Participant_001_Rep1
BioProject PRJNA472585

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114763_RAW.tar 721.8 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

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