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Series GSE114737 Query DataSets for GSE114737
Status Public on Dec 27, 2018
Title Optimized ChIP-seq procedure facilitates hormone receptor profiling in human tumors
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Performing ChIP-seq analyses in clinical specimens has remained largely challenging due to multiple technical limitations and low quantities of starting material, resulting in low enrichments and poor signal-to-noise ratio. Here, we refined the original protocols for transcription factor ChIP-seq analyses in breast, prostate, and endometrial tumor tissue. In addition to the standard fixative formaldehyde, a second crosslinker Disuccinimidyl glutarate (DSG) was included in the procedure.
 
Overall design AR, FOXA1, ERĪ±, H3K27ac & H3K4me3 ChIP-seq data with and without double croslinking in celllines (LNCAP & MCF-7) and in human tissues (Prostate, Breast, Endometrium and Prostate samples from core needle biopsies).
 
Contributor(s) Zwart W, Singh AA, Stello S, Schuurman K, Linder S, Droog M
Citation(s) 30620009
Submission date May 21, 2018
Last update date Sep 30, 2019
Contact name Abhishek Singh
E-mail(s) abhisheksinghnl@gmail.com
Organization name Netherlands Cancer Institute
Street address Plesmanlaan
City Amsterdam
ZIP/Postal code 1066CX
Country Netherlands
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (99)
GSM3149022 LNCAP_AR_FA
GSM3149023 LNCAP_AR_DSG
GSM3149024 LNCAP_FOXA1_FA
Relations
BioProject PRJNA472620
SRA SRP148757

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114737_RAW.tar 15.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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