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Series GSE114572 Query DataSets for GSE114572
Status Public on Oct 03, 2018
Title PyMT-1099, a murine derived cell line is a breast cancer specific model for EMT
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: Epithelial-mesenchymal transition (EMT) has been implicated in metastasis, drug resistance, survival under stress and also conferring stem cell-like traits to cancer cells. However, several of the studies have been carried out using model systems that don’t appropriately recapitulate all stages of the dynamic process of EMT. Hence, there is a need to overcome this limitation by development of a model system that allows us to mimic each stage of EMT and accurately assess the plastic changes associated with it. Methods: We have derived a cancer cell line from the PyMT-MMTV model of breast cancer, named PyMT-1099 cells, and undertaken a detailed characterization of the morpho-genetic changes it undergoes during a TGF-induced EMT. Further, we have also performed high throughput transcriptomics on PyMT-1099 cells undergoing EMT in a high resolution kinetic of TGF treatment. Results: We show that PyMT-1099 cells undergo an EMT comparable to the classically used immortalized NMuMG cells as assessed by morphological, and marker expression changes on TGFtreatment. Further, PyMT-1099 cells can also migrate in vitro in response to TGF treatment. These cells are also tumorigenic and lead to metastasis formation when transplanted into immunocompromised mice. Conclusion: In this study we report the development of PyMT-1099 cells as an excellent tool to model and study breast cancer-associated EMT both in vitro and in vivo and show that these cells overcome the limitations posed by other cellular systems currently being used to study EMT
Overall design RNA-Seq of TGFb-induced EMT and MET over a timecourse of 10 days in PyMT-1099 cells performed in biological duplicates
Contributor(s) Saxena M, Kalathur RK, Christofori G
Citation(s) 30108334
Submission date May 17, 2018
Last update date Mar 25, 2019
Contact name Ravi Kiran Reddy KALATHUR
Organization name University of Basel
Department Departement Biomedizin
Street address Mattenstrasse 28
City Basel
State/province Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (38)
GSM3145140 1099_emt_ut_replicate_1
GSM3145141 1099_emt_2h_replicate_1
GSM3145142 1099_emt_6h_replicate_1
BioProject PRJNA471818
SRA SRP148095

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Supplementary file Size Download File type/resource
GSE114572_1099_EMT_CPM_data.txt.gz 3.2 Mb (ftp)(http) TXT
GSE114572_1099_MET_CPM_data.txt.gz 1.6 Mb (ftp)(http) TXT
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