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| Status |
Public on Jun 21, 2018 |
| Title |
Determination of global decay rates of yeast transcriptome and identification of factors impact mRNA stability |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this work, we determine total mRNA decay rates in rpb1-1 and rpb1-1/caf1∆ cells, calculate half-lives in rpb1-1/caf1∆ cells relative to rpb1-1 cells and correlate them with codon optimality.
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| Overall design |
mRNA profiling was done on 10 time points in rpb1-1/caf1 cells and sequenced using a paired end protocol on an Illumina HiSeq2000 instrument. A biological duplicate was performed.
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| |
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| Citation(s) |
29932902 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM118018 |
Determining the influence of codon optimality on mRNA translation and decay |
CASE WESTERN RESERVE UNIVERSITY |
Jeffery Coller |
| R01 GM125086 |
Understanding the Interconnection Between mRNA turnover and mRNA Translation |
CASE WESTERN RESERVE UNIVERSITY |
Jeffery Coller |
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| |
| Submission date |
May 16, 2018 |
| Last update date |
Mar 13, 2019 |
| Contact name |
Jeff Coller |
| E-mail(s) |
jmc71@case.edu
|
| Phone |
216-368-0299
|
| Organization name |
Case Western Reserve University
|
| Department |
Center for RNA Science and Therapeutics
|
| Street address |
10900 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA471740 |
| SRA |
SRP147931 |
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