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Series GSE11452 Query DataSets for GSE11452
Status Public on Feb 05, 2009
Title Saccharomyces cerevisiae chemostat steady state microarray compendium
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Background
Microorganisms adapt their transcriptome by integrating multiple chemical and physical signals from their environment. Shake-flask cultivation does not allow precise manipulation of individual culture parameters and therefore precludes a quantitative analysis of the (combinatorial) influence of these parameters on transcriptional regulation. Steady-state chemostat cultures, which do enable accurate control, measurement and manipulation of individual cultivation parameters (e.g. specific growth rate, temperature, identity of the growth-limiting nutrient) appear to provide a promising experimental platform for such a combinatorial analysis.
Results
A microarray compendium of 170 steady-state chemostat cultures of the yeast Saccharomyces cerevisiae is presented and analyzed. The 170 microarrays encompass 55 unique conditions, which can be characterized by the combined settings of 10 different cultivation parameters.
By applying a regression model to assess the impact of (combinations of) cultivation parameters on the transcriptome, most S. cerevisiae genes were shown to be influenced by multiple cultivation parameters, and in many cases by combinatorial effects of cultivation parameters. The inclusion of these combinatorial effects in the regression model led to higher explained variance of the gene expression patterns and resulted in higher function enrichment in subsequent analysis.
We further demonstrate the usefulness of the compendium and regression analysis for interpretation of shake-flask-based transcriptome studies and for guiding functional analysis of (uncharacterized) genes and pathways.
Conclusions
Modeling the combinatorial effects of environmental parameters on the transcriptome is crucial for understanding transcriptional regulation. Chemostat cultivation offers a powerful tool for such an approach.
Keywords: chemostat steady state samples
 
Overall design The Saccharomyces cerevisiae laboratory reference strain CEN.PK 113-7D MATa was grown at steady state in chemostat cultures under 55 different conditions. A condition can be characterized by a specific configuration of the settings of ten different cultivation parameters. One of these cultivation parameters is the available carbon source. Throughout the compendium five different carbon sources were used, i.e. acetate, ethanol,, galactose, glucose and maltose. Other paramaters as Aeration type, Medium type, C-source(s), N-source, S-source, P-source, Limiting element, Growth rate, Temperature (C), pH, Extra compound, Protocol can be analysed.
 
Contributor(s) Knijnenburg TA, Daran J, van den Broek MA, Daran-Lapujade P, de Winde JH, Pronk JT, Reinders MJ, Wessels LF
Citation(s) 19173729
Submission date May 15, 2008
Last update date Jul 01, 2016
Contact name Jean-Marc Daran
E-mail(s) j.g.daran@tudelft.nl
Phone +31 15 278 2412
Organization name Delft University of Technology
Department Department of Biotechnology
Lab Kluyver centre for genomics of industrial organisms
Street address Julianalaan 67
City Delft
ZIP/Postal code 2628BC
Country Netherlands
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (170)
GSM29912 Aerobic glucose limited 1
GSM29914 Aerobic glucose limited 2
GSM29917 Aerobic glucose limited 3
Relations
BioProject PRJNA106367

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11452_RAW.tar 358.6 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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