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Series GSE11450 Query DataSets for GSE11450
Status Public on Jan 12, 2009
Title Transcriptome analysis of human mature oocytes and embryonic stem cells reveals overexpression of the proteasome pathway
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The first week of human pre-embryo development is characterized by the induction of totipotency and then pluripotency. The understanding of this delicate process will have far reaching implication for in vitro fertilization and regenerative medicine. Human mature MII oocytes and embryonic stem (ES) cells are both able to achieve the feat of cell reprogramming towards pluripotency, either by somatic cell nuclear transfer or by cell fusion, respectively. Comparison of the transcriptome of these two cell types may highlight genes that are involved in pluripotency initiation. Therefore, based on a microarray compendium of 205 samples, produced in our laboratory or from public databases, we compared the gene expression profile of mature MII oocytes and human ES cells (hESC) to that of somatic tissues. We identified a common oocyte/hESC gene expression profile, which included a strong cell cycle signature, a large chromatin remodelling network (TOP2A, DNMT3B, JARID2, SMARCA5, CBX1, CBX5) and 18 different zinc finger transcription factors, including ZNF84. Strikingly, a large set of genes was found to code for proteins involved in the ubiquitination and proteasome pathway. Upon hESC differentiation into embryoid bodies, the transcription of this pathway declines. In vitro, we observed a selective sensitivity of hESC to the inhibition of the activity of the proteasome, resulting in loss of pluripotency and cell growth at doses without any detectable effects on differentiated cells. Taken together, these results suggest that the proteasome pathway may play a role in initiating and maintaining pluripotency during early development and in hESC.
Keywords: cell type comparison
 
Overall design We included in this study 9 samples obtained in our laboratory. These samples were hybridized on U133 Plus 2.0 GeneChips (Affymetrix). All samples were normalized using the MAS5 (GCOS 1.2) algorithm, using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
Human embryonic stem cells (hESC) samples were compared to somatic tissues, human mature oocytes samples were compared to somatic tissues, then the hESC and the mature oocyte signatures were interesected to define an oocyte/hESC signature.
 
Contributor(s) DE VOS J, ASSOU S, HAMAMAH S
Citation(s) 19128516
Submission date May 15, 2008
Last update date Mar 25, 2019
Contact name John De Vos
E-mail(s) john.devos@inserm.fr
Organization name University Hospital of Montpellier
Department Institute for Research in Biotherapy
Lab Microarray Core Facility
Street address 80 av. Augustin Fliche
City Montpellier
ZIP/Postal code 34000
Country France
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (9)
GSM288812 Oocyte_M2-21
GSM288876 Oocyte_M2-24
GSM288877 Oocyte_M2-16
Relations
BioProject PRJNA106363

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11450_RAW.tar 43.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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