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Series GSE114241 Query DataSets for GSE114241
Status Public on May 10, 2018
Title Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We compared the genome occupancy for FLAG-tagged versions of the ETS factors ERG and EHF in the normal prostate epithelial cell line RWPE1. Our in vitro binding studies support a model whereby oncogenic ETS factors like ERG bind cooperativly with AP1 factors at closly spaced ETS-AP1 sites, while certain non-oncogenic factors like EHF bind anti-cooperatively with AP1 at the same sites. ETS and AP1 binding motifs were enriched in both ChIP datasets, but the ERG-FLAG bound reginos contained a much higher percentage of ETS-AP1 sites spaced in close proximity, consistent with our in vitro binding data.
Overall design ChIP-seq with anti-FLAG antibody in RWPE1 cells expressing either FLAG-ERG or FLAG-EHF
Contributor(s) Clark K, Graves B
Citation(s) 30315111
Submission date May 09, 2018
Last update date Mar 27, 2019
Contact name Kathleen Clark
Phone 801-809-7635
Organization name University of Utah
Department Oncological Sciences
Street address 2000 Circle of Hope
City Salt Lake City
State/province UT
ZIP/Postal code 84108
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (3)
GSM3138381 input
BioProject PRJNA470652
SRA SRP145081

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Supplementary file Size Download File type/resource
GSE114241_RAW.tar 286.9 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data are available on Series record

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