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Series GSE114241 Query DataSets for GSE114241
Status Public on May 10, 2018
Title Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We compared the genome occupancy for FLAG-tagged versions of the ETS factors ERG and EHF in the normal prostate epithelial cell line RWPE1. Our in vitro binding studies support a model whereby oncogenic ETS factors like ERG bind cooperativly with AP1 factors at closly spaced ETS-AP1 sites, while certain non-oncogenic factors like EHF bind anti-cooperatively with AP1 at the same sites. ETS and AP1 binding motifs were enriched in both ChIP datasets, but the ERG-FLAG bound reginos contained a much higher percentage of ETS-AP1 sites spaced in close proximity, consistent with our in vitro binding data.
 
Overall design ChIP-seq with anti-FLAG antibody in RWPE1 cells expressing either FLAG-ERG or FLAG-EHF
 
Contributor(s) Clark K, Graves B
Citation(s) 30315111
Submission date May 09, 2018
Last update date Mar 27, 2019
Contact name Kathleen Clark
E-mail(s) kathleen.clark@hci.utah.edu
Phone 801-809-7635
Organization name University of Utah
Department Oncological Sciences
Street address 2000 Circle of Hope
City Salt Lake City
State/province UT
ZIP/Postal code 84108
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (3)
GSM3138379 FLAG-ERG_ChIPSeq
GSM3138380 FLAG-EHF_ChIPSeq
GSM3138381 input
Relations
BioProject PRJNA470652
SRA SRP145081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114241_RAW.tar 286.9 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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