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Series GSE114032 Query DataSets for GSE114032
Status Public on Jul 31, 2018
Title Sperm Epimutation Biomarkers of Obesity and Pathologies following DDT Induced Epigenetic Transgenerational Inheritance of Disease
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary Gestating F0 generational female rats were transiently exposed to DDT during fetal gonadal sex determination and the incidence of adult onset pathologies was assessed in the subsequent F1, F2, and F3 generations. In addition, sperm differential DNA methylation regions (DMRs) that were associated with specific pathologies in the F3 generation males were investigated. No pathology was observed in the F1 generation DDT lineage males or females compared with F1 generation controls (vehicle exposure). There was an increase of testis disease and early onset puberty in the F2 generation DDT lineage males. The F3 generation DDT males had significant increases in testis disease, prostate disease, and late onset puberty. The F3 generation DDT females had significant increases in ovarian and kidney disease. Both the F3 generation males and females had significant increases in the frequency of obesity and multiple disease. The F3 generation sperm was collected to examine DMRs for the ancestrally exposed DDT male population. Unique sets of DMRs were associated with late onset puberty, kidney disease, and multiple disease pathologies.
Overall design Outbred gestating female rats were transiently exposed to a vehicle control or DDT The F1 generation offspring were bred to generate the F2 generation and then the F2 generation bred to generate the F3 generation. The F3 generation control and DDT lineage rats were aged and various pathologies investigated. The male sperm were collected to investigate DNA methylation differences between the DDT lineage sperm from samples with different disease outcomes. MeDIP-seq was used to measure methylation in each sample.
Contributor(s) King S, McBirney M, Beck D, Sadler-Riggleman I, Nilsson E, Skinner MK
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Submission date May 04, 2018
Last update date Oct 22, 2018
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (40)
GSM3131001 T1
GSM3131002 T2
GSM3131003 T3
BioProject PRJNA459562
SRA SRP144581

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Supplementary file Size Download File type/resource
GSE114032_combined.results.csv.gz 1.8 Gb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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