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Series GSE113913 Query DataSets for GSE113913
Status Public on May 02, 2018
Title Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic stem cells (ESCs) maintain pluripotency through unique epigenetic states. When ESCs commit to a specific cell lineage, changes in histone modifications and DNA methylation accompany the transition to more specialized cell types. Investigating how epigenetic regulation maintains pluripotency is a critical component in order to generate the required cell types for future clinical application. Uhrf1 is a widely known hemi-methylated DNA binding protein, playing a role in heterochromatin formation alongside G9a, Trim28 and HDACs. In addition, it has been demonstrated that Uhrf1 functions to maintain DNA methylation through the recruitment of Dnmt1. Although Uhrf1 is not essential in ESC self-renewal, it still remains elusive how Uhrf1 regulates pluripotency. Here, we set out to elucidate the role of Uhrf1 in pluripotent stem cells. We found that Uhrf1 forms a complex with the active trithorax group of transcriptional regulators, in particular the Setd1a/COMPASS complex. Our data show that loss of Uhrf1 causes a dramatic reduction of bivalent histone marks, in particular those associated with neuroectoderm and mesoderm specification. Overall, our data demonstrate that Uhrf1 safeguards the balance between pluripotency and differentiation via the Setd1a/COMPASSS complex, through which Uhrf1 mediates bivalent histone modifications.
Overall design Genome-wide distribution of Uhrf1, histone modifications and core pluripotent factors of WT and Uhrf1 KO mESCs and EBs
Contributor(s) Kim K, Tanaka Y, Park I
Citation(s) 29968706
Submission date May 01, 2018
Last update date Mar 19, 2019
Contact name Kun-Yong Kim
Phone 7344782979
Organization name Yale University
Department Genetics
Street address 10 Amistad Street
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (33)
GSM3123475 WT mESC H3K4me3
GSM3123476 WT EB week1 H3K4me3
GSM3123477 WT EB week2 H3K4me3
This SubSeries is part of SuperSeries:
GSE113915 Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a
BioProject PRJNA454460
SRA SRP144193

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Supplementary file Size Download File type/resource
GSE113913_RAW.tar 4.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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