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Series GSE11386 Query DataSets for GSE11386
Status Public on Jun 05, 2008
Title Gene expression in murine memory versus naive B cells (1st generation and 2nd generation screens)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Memory B cells play essential roles in the maintenance of long-term immunity and may be important in the pathogenesis of autoimmune disease, but how these cells are distinguished from their naïve precursors is poorly understood. To address this, it would be important to understand how gene expression differs between memory and naive B cells in order to elucidate memory-specific functions. Using model systems that help overcome the lack of murine memory-specific markers and the low frequency of antigen-specific memory and naïve cells, we undertook a global comparison of gene expression between memory B cells and their naive precursors.
1st generation screen:
These data represent our first generation comparison of gene expression between murine naïve and memory splenic B cells. Naïve NP-binding splenic B cells were FACS purified from unimmunized mVh186.2 transgenic Balb/c mice. Memory B cells were generated by immunizing mVh186.2 transgenic Balb/c mice with 2 doses of NP-CGG in alum delivered i.p. 6 weeks apart. After a minimum of 12-weeks rest, NP-binding splenic B cells were isolated by FACS. Total RNA was extracted, cRNA synthesized and labeled and hybridized to Affymetrix mouse 430 2.0 chips.
2nd generation screen:
These data represent our second generation comparison of gene expression between murine naïve and memory splenic B cells. Naïve NP-binding AA4.1neg splenic B cells were FACS purified from unimmunized mVh186.2 transgenic Jk KO Balb/c mice. Memory B cells were generated from these naive precursors after adoptive transfer into recipients that mount poor endogenous NP-responses. 12-weeks post i.p. immunization with NP-CGG in alum, NP-specific splenic memory B cells were isolated by FACS. Total RNA was extracted, cRNA synthesized and labeled and hybridized to Affymetrix mouse 430 2.0 chips.

Memory/Naïve comparison data linked below as Supplementary files.

Keywords: Cell type comparison
 
Overall design 1st generation screen: 7 samples, 3-4 biological replicates each of murine splenic naïve and memory B cells
2nd generation screen: 8 samples, 4 biological replicates each of murine splenic naïve and memory B cells
 
Contributor(s) Tomayko MM, Shlomchik MJ
Citation(s) 18566367
Submission date May 08, 2008
Last update date Feb 11, 2019
Contact name Mary M. Tomayko
E-mail(s) mary.tomayko@yale.edu
Phone 203-785-7349
Fax 203-785-5415
Organization name Yale University
Department Dermatology
Lab Mark J. Shlomchik
Street address PO BOX 208059
City New Haven
State/province CT
ZIP/Postal code 06520-8059
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (15)
GSM287567 Naive B cell (A)_1st generation screen
GSM287568 Naive B cell (B)_1st generation screen
GSM287569 Naive B cell (C)_1st generation screen
Relations
BioProject PRJNA106505

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11386_1st_generation_screen_suppl.txt 3.4 Mb (ftp)(http) TXT
GSE11386_2nd_generation_screen_suppl.txt 4.2 Mb (ftp)(http) TXT
GSE11386_RAW.tar 91.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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