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Series GSE113785 Query DataSets for GSE113785
Status Public on Jul 25, 2018
Title Vinclozolin induced epigenetic transgenerational inheritance of pathologies and sperm epimutation biomarkers for specific diseases
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary F0 generation rats were exposed to vinclozolin, and the subsequent F1, F2 and transgenerational F3 generations are evaluated for diseases and pathologies. F3 generation rats showed transgenerational increases in testis, prostate, and kidney disease, changes in the age of puberty onset in males, and an increased obesity rate in females. Overall there was an increase in the rate of animals with disease, and in the incidence of animals with multiple disease. The methylation of the F3 vinclozolin lineage rat samples was determined using MeDIP-seq. Unique signatures of differential DNA methylation regions (DMRs) in sperm were found that associated with testis disease, prostate disease and kidney disease, however, these signatures were not statistically significant.
Overall design Outbred gestating female rats were transiently exposed to a vehicle control or vinclozolin. The F1 generation offspring were bred to generate the F2 generation and then the F2 generation bred to generate the F3 generation. The F3 generation control and atrazine lineage rats were aged and various pathologies investigated. The male sperm were collected to investigate DNA methylation differences between the vinclozolin lineage sperm from samples with different disease outcomes. MeDIP-seq was used to measure methylation in each sample.
Contributor(s) Nilsson E, King SE, McBirney M, Kubsad D, Pappalardo M, Beck D, Sadler-Riggleman I, Skinner MK
Citation(s) 30157260
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 ES012974 Epigenetic Transgenerational Endocrine Disruptor Actions Washington State University MICHAEL K SKINNER
Submission date Apr 27, 2018
Last update date Oct 24, 2018
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (27)
GSM3119367 S1
GSM3119368 S2
GSM3119369 S3
BioProject PRJNA453994
SRA SRP143493

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Supplementary file Size Download File type/resource
GSE113785_combined.results.csv.gz 1.4 Gb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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