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| Status |
Public on Dec 14, 2018 |
| Title |
H3K27ac profiling of human tracheobronchial epithelial cells during infection with H1N1, H3N2, and H5N1 influenza virus [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. ChIP-Seq analysis was used to profile histone acetylation (H3K27ac) in HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
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| Overall design |
Human tracheobronchial epithelial cells were infected with H1N1, H3N2, and H5N1 influenza virus at an MOI of 5. H3N2- and H5N1-infected samples were collected in duplicates at 3, 6, 12, and 18 hours post infection for ChIP-Seq analysis. Sample from H1N1-infected cells were collected at 6, 12, 18, and 24 hours. Uninfected controls (mock infection) were also collected at a single time.
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| Contributor(s) |
Urbanowski M, Shaw M, Rialdi A, Marazzi I, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 26, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Max Chang |
| E-mail(s) |
mchang@ucsd.edu
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| Organization name |
University of California, San Diego
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (52)
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| Relations |
| BioProject |
PRJNA453842 |
| SRA |
SRP143484 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113702_RAW.tar |
7.8 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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