GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE113702 Query DataSets for GSE113702
Status Public on Dec 14, 2018
Title H3K27ac profiling of human tracheobronchial epithelial cells during infection with H1N1, H3N2, and H5N1 influenza virus [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. ChIP-Seq analysis was used to profile histone acetylation (H3K27ac) in HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
Overall design Human tracheobronchial epithelial cells were infected with H1N1, H3N2, and H5N1 influenza virus at an MOI of 5. H3N2- and H5N1-infected samples were collected in duplicates at 3, 6, 12, and 18 hours post infection for ChIP-Seq analysis. Sample from H1N1-infected cells were collected at 6, 12, 18, and 24 hours. Uninfected controls (mock infection) were also collected at a single time.
Contributor(s) Urbanowski M, Shaw M, Rialdi A, Marazzi I, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 26, 2018
Last update date Mar 26, 2019
Contact name Max Chang
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (52)
GSM3112317 HTBE_H1N1_06h_H3K27ac_1
GSM3112318 HTBE_H1N1_06h_H3K27ac_2
GSM3112319 HTBE_H1N1_06h_Input_1
BioProject PRJNA453842
SRA SRP143484

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113702_RAW.tar 7.8 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap