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Series GSE113702 Query DataSets for GSE113702
Status Public on Dec 14, 2018
Title H3K27ac profiling of human tracheobronchial epithelial cells during infection with H1N1, H3N2, and H5N1 influenza virus [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. ChIP-Seq analysis was used to profile histone acetylation (H3K27ac) in HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
 
Overall design Human tracheobronchial epithelial cells were infected with H1N1, H3N2, and H5N1 influenza virus at an MOI of 5. H3N2- and H5N1-infected samples were collected in duplicates at 3, 6, 12, and 18 hours post infection for ChIP-Seq analysis. Sample from H1N1-infected cells were collected at 6, 12, 18, and 24 hours. Uninfected controls (mock infection) were also collected at a single time.
 
Contributor(s) Urbanowski M, Shaw M, Rialdi A, Marazzi I, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
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Submission date Apr 26, 2018
Last update date Mar 25, 2019
Contact name Max Chang
E-mail mchang@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (52)
GSM3112317 HTBE_H1N1_06h_H3K27ac_1
GSM3112318 HTBE_H1N1_06h_H3K27ac_2
GSM3112319 HTBE_H1N1_06h_Input_1
Relations
BioProject PRJNA453842
SRA SRP143484

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113702_RAW.tar 7.8 Gb (http)(custom) TAR (of BW)
Raw data are available in SRA
Processed data provided as supplementary file

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