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Series GSE113573 Query DataSets for GSE113573
Status Public on Jun 05, 2018
Title DHX15 regulates CMTR1-dependent gene expression and cell proliferation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We discovered that the RNA helicase DHX15 is a regulator of the methyltransferase CMTR1. To determine the biological consequences of this interaction we overexpressed CMTR1 or a mutated CMTR1 (2LA)that does not bind DHX15, in the human breast cancer cell line HCC1806. To assess the effects of the DHX15-CMTR1 interaction on translation, polysomes were separated on a sucrose gradient and sequencing libraries generated from the polysomal and input RNA. We found that the DHX15-CMTR1 interaction controls ribosome loading of a subset of mRNAs and impacts on cell proliferation.
Overall design Polysome and input RNA samples were sequenced from two replicate experiments. In each experiment HCC1806 cells were transfected with either pcDNA5 (vector control), pcDNA 5 HA-CMTR1 WT (wildtype CMTR1) or pcDNA 5 HA-CMTR1 2L/A mutant (mutant CMTR1 that does not interact with DHX15).
Contributor(s) Inesta-Vaquera F, Galloway A, Cowling VH
Citation(s) 30079402
Submission date Apr 24, 2018
Last update date Mar 26, 2019
Contact name Alison Galloway
Organization name University of Dundee
Department Centre for Gene Regulation and Expression
Lab Victoria Cowling
Street address Life sciences, Dow street
City Dundee
State/province Angus
ZIP/Postal code DD1 5EH
Country United Kingdom
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM3109233 Input vector #1
GSM3109234 Input CMTR1 #1
GSM3109235 Input CMTR1 2/LA #1
BioProject PRJNA453131
SRA SRP142438

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Supplementary file Size Download File type/resource
GSE113573_2LA_vs_Vec_Input_edgeR_analysis.csv.gz 1.2 Mb (ftp)(http) CSV
GSE113573_2LA_vs_Vec_Polys_edgeR_analysis.csv.gz 1.1 Mb (ftp)(http) CSV
GSE113573_WT_vs_2LA_Input_edgeR_analysis.csv.gz 1.2 Mb (ftp)(http) CSV
GSE113573_WT_vs_2LA_Polys_edgeR_analysis.csv.gz 1.1 Mb (ftp)(http) CSV
GSE113573_WT_vs_Vec_Input_edgeR_analysis.csv.gz 1.2 Mb (ftp)(http) CSV
GSE113573_WT_vs_Vec_Polys_edgeR_analysis.csv.gz 1.0 Mb (ftp)(http) CSV
GSE113573_read_count_table.csv.gz 619.1 Kb (ftp)(http) CSV
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