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| Status |
Public on Jun 27, 2018 |
| Title |
PARCLIP analysis of NONO and SFPQ from U2OS and Hela cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
To identify the RNA targets of the RNA binding proteins NONO and SFPQ in two different cell types, PARCLIP was carried out (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) using a monoclonal antibody that recognises a NONO/SFPQ heterodimer. In this analysis, only the target gene NEAT1 was analysed, to show the binding sites for NONO and SFPQ in this transcript in the two cell lines.
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| Overall design |
HeLa and U2OS cells were cultured and crosslinked according to the PARCLIP protocol. An antibody that pulls down the NONO/SFPQ heterodimer was used to purify these specific complexes. One replicate sample in each case was sequenced and analysed. Analysis of Neat1_2 regions that bind the paraspeckle proteins NONO and SFPQ was performed
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| Contributor(s) |
Fox A, Kobelke S, Yamazaki T |
| Citation(s) |
29932899 |
| |
| Submission date |
Apr 18, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Archa Fox |
| E-mail(s) |
archa.fox@uwa.edu.au
|
| Organization name |
University of Western Australia
|
| Street address |
35 Stirling Highway
|
| City |
Nedlands |
| ZIP/Postal code |
6009 |
| Country |
Australia |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA450858 |
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