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Series GSE113036 Query DataSets for GSE113036
Status Public on Jun 05, 2018
Title Pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Ectopic expression of defined transcription factors can force direct cell fate conversion from one lineage to another in the absence of cell division. Several transcription factor cocktails have enabled successful reprogramming of various somatic cell types into induced neurons (iNs) of distinct neurotransmitter phenotype. However, the nature of the intermediate states that drive the reprogramming trajectory towards distinct iN types is largely unknown. Here we show that successful direct reprogramming of adult human brain pericytes into functional iNs by Ascl1 and Sox2 (AS) encompasses transient activation of a neural stem cell-like gene expression program that precedes bifurcation into distinct neuronal lineages. Intriguingly, during this transient state key signaling components relevant for neural induction and neural stem cell maintenance are regulated and functionally contribute to iN reprogramming and maturation. Thus, AS-mediated reprogramming into a broad spectrum of iN types involves the unfolding of a developmental program via neural stem cell-like intermediates.
Overall design Single-cell transcriptomes from multiple time points and conditions during direct conversion of human pericytes into induced pericytes through the overexpression of defined factors.

Please note that [1] the *ctrl samples represent mock-transfected cells (analyzed along side of the transfected cells) [2] The cell type (for each sample) is provided as 'pericytes or pericyte-derived induced neuronal cells' (as they are in a differentiation continuum from pericytes to neurons due to the treatment protocol) with the combination of 'genotype/variation' and 'time point' information.
Contributor(s) Camp JG, Treutlein B
Citation(s) 29915193
Submission date Apr 12, 2018
Last update date Mar 27, 2019
Contact name Gray Camp
Organization name Max Planck Institute for Evolutionary Anthropology
Department Evolutionary Genetics
Street address Deutscher Pl. 6
City Leipzig
ZIP/Postal code 04103
Country Germany
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (770)
GSM3135891 H9B_ctrl_d2
GSM3135892 H8B_ctrl_d2
GSM3135893 H7B_ctrl_d2
BioProject PRJNA464430
SRA SRP144912

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Supplementary file Size Download File type/resource
GSE113036_RAW.tar 65.8 Mb (http)(custom) TAR (of CSV, MTX, TSV)
GSE113036_data.master.csv.gz 32.1 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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