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Series GSE112951 Query DataSets for GSE112951
Status Public on Aug 31, 2018
Title DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis [ChIP-seq]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates the destabilization of key regulators of cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis that DET1 is essential for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of a plant deubiquitination module (DUBm). In contrast with yeast and metazoan DUB modules that are associated with the large SAGA complex, the Arabidopsis DUBm only comprises three proteins (hereafter named SGF11, ENY2 and UBP22) and appears to act independently as a major H2Bub deubiquitinase activity. Our study further unveils that DET1-DDB1-Associated-1 (DDA1) protein interacts with SGF11 in vivo, linking the DET1 complex to light-dependent ubiquitin-mediated proteolytic degradation of the DUBm. Collectively, these findings uncover a signaling path controlling DUBm availability, potentially adjusting H2Bub turnover capacity to the cell transcriptional status.
 
Overall design 16 ChIP-seq libraries were sequenced.
 
Contributor(s) Bourbousse C, Ait-Mohamed O, Rougée M, Barneche F
Citation(s) 30192741
Submission date Apr 10, 2018
Last update date Dec 04, 2018
Contact name Ouardia Ait_Mohamed
E-mail(s) amohamed@biologie.ens.fr
Phone +33144323527
Organization name ENS
Department IBENS
Lab Génomique des Plantes et Algues
Street address 46 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (16)
GSM3092008 WT_L_rep1_input
GSM3092009 WT_L_rep2_input
GSM3092010 WT_D_rep1_input
This SubSeries is part of SuperSeries:
GSE112952 DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis
Relations
BioProject PRJNA449910
SRA SRP139782

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112951_RAW.tar 481.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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