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| Status |
Public on Mar 23, 2019 |
| Title |
Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We investigated the nuclear topography, organization and genome-wide gH2AX distribution of unrepaired DNA in rat cortical neurons 15 dyas upon X-Ray ionizing adiation (4Gy). Unrepaired DNA is confined in persistent DNA damage foci which would help preserving genome integrity. ChIp-seq analysis revealed a number of genomic regions enriched in gH2AX. Some of these regions are in close proximity to genes encoding essential proteins for neuronal function and human neurodegenerative disorders.
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| Overall design |
ChIP-seq analysis of genome-wide gH2AX distribution in persistent DNA damage foci of unrepaired DNA in both irradiated and non-irradiated cortical neurons
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| Contributor(s) |
Mata-Garrido J, Tapia O, Casafont I, Berciano MT, Cuadrado A, Lafarga M |
| Citation missing |
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| |
| Submission date |
Apr 10, 2018 |
| Last update date |
Mar 24, 2019 |
| Contact name |
Miguel Angel Lafarga |
| E-mail(s) |
miguel.lafarga@unican.es
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| Phone |
34 942201929
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| Organization name |
Faculty of Medicine
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| Department |
Department of Anatomy and Cell Biology
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| Street address |
Avd,. Cardenal Herrera Oria sn
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| City |
Santander |
| ZIP/Postal code |
39011 |
| Country |
Spain |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA449523 |
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