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| Status |
Public on Apr 06, 2018 |
| Title |
Piwi knockdown in Drosophila Intestinal Stem Cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome analysis of Piwi-deficient ISCs isolated by FACS. We used esg::Gal4, UAS::GFP; tub::Gal80ts to drive the expression of an RNAi against mCherry (control) or Piwi for 7 days.
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| Overall design |
Duplicates of mCherry RNAi expressing ISCs and Piwi RNAi expressing ISCs. Each sample was obtained from a pool of 100 dissected and FACSorted fly guts.
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| Contributor(s) |
Sousa-Victor P, Jasper H |
| Citation missing |
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| |
| Submission date |
Apr 05, 2018 |
| Last update date |
Feb 20, 2019 |
| Contact name |
Pedro Sousa-Victor |
| E-mail(s) |
pvictor@buckinstitute.org
|
| Phone |
4152023045
|
| Organization name |
Buck Institute for Research on Aging
|
| Lab |
Jasper Lab
|
| Street address |
8001 Redwood Blvd.
|
| City |
Novato |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA448884 |
| SRA |
SRP137690 |
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