 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 04, 2018 |
| Title |
Alteration of TAC1 expression in Prunus species leads to pleiotropic shoot phenotypes |
| Organism |
Prunus persica |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Prunus persica (peach) trees carrying the ‘Pillar’ or ‘Broomy’ trait (br) have vertically oriented branches caused by loss of function mutations in a gene called TILLER ANGLE CONTROL 1 (TAC1). TAC1 encodes a protein in the IGT gene family that includes LAZY1 and DEEPER ROOTING 1 (DRO1), which regulat lateral branch and root orientations, respectively. Here, we found that some of the native TAC1 alleles in the hexaploid plum species Prunus domestica, which has a naturally more upright stature, contained a variable length trinucleotide repeat within the same exon 3 region previously found to be disrupted in pillar peach trees. RNAi silencing of TAC1 in plum resulted in trees with severely vertical branch orientations similar to those in pillar peaches but with an even narrower profile. In contrast, PpeTAC1 over-expression in plum led to trees with wider branch angles and more horizontal branch orientations. Pillar peach trees and transgenic plum lines exhibited pleiotropic phenotypes including differences in trunk and branch diameter, stem growth, and twisting branch phenotypes. Expression profiling of pillar peach trees revealed differential expression of numerous genes associated with biotic and abiotic stress, hormone responses, plastids, reactive oxygen, and secondary and cell wall metabolism. Collectively, the data provide important clues for understanding TAC1 function and show that alteration of TAC1 expression may have broad applicability to agricultural and ornamental tree industries.
|
| |
|
| Overall design |
mRNA was isolated from four shoot tips (approximately 4cm long) from standard and pillar peach trees. Four biological replicates for each type of tree were sequenced using illumina Hiseq2000
|
| |
|
| Contributor(s) |
Hollender C, Dardick C |
| Citation(s) |
29736251 |
| |
| Submission date |
Apr 03, 2018 |
| Last update date |
Aug 21, 2018 |
| Contact name |
Courtney Hollender |
| E-mail(s) |
chollend@msu.edu
|
| Phone |
517-353-0446
|
| Organization name |
Michigan State University
|
| Department |
Dept. of Horticulture
|
| Lab |
Hollender Lab
|
| Street address |
1066 Bogue St
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15671 |
Illumina HiSeq 2000 (Prunus persica) |
|
| Samples (8)
|
| GSM3074902 |
Sequencing data from the shoot tips of one standard peach branch - standard tree #7 |
| GSM3074903 |
Sequencing data from the shoot tips of one standard peach branch - standard tree #8 |
| GSM3074904 |
Sequencing data from the shoot tips of one standard peach branch - standard tree #9 |
| GSM3074905 |
Sequencing data from the shoot tips of one standard peach branch - standard tree #10 |
| GSM3074906 |
Sequencing data from the shoot tips of one pillar peach branch - pillar tree #4 |
| GSM3074907 |
Sequencing data from the shoot tips of one pillar peach branch - pillar tree #7 |
| GSM3074908 |
Sequencing data from the shoot tips of one pillar peach branch - pillar tree #8 |
| GSM3074909 |
Sequencing data from the shoot tips of one pillar peach branch - pillar tree #10 |
|
| Relations |
| BioProject |
PRJNA448600 |
| SRA |
SRP137017 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112649_TAC_tree_RNAseq_processed_data_CLC_DEG_output.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...