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Series GSE112607 Query DataSets for GSE112607
Status Public on Apr 02, 2019
Title To determine the potential molecular mechanisms by which STAT5 signaling controls colonic crypt homeostasis, we isolated total RNA from ileal intact crypts of STAT5+/+, STAT5DIEC-/- and STAT5DIEC+++ mice and performed RNA sequencing (RNA-seq).
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Reads were aligned to the mm10 genome with annotations provided by Ensembl. Transcripts were filtered, requiring at least 3 reads in 50% of samples within at least one condition. To identify differentially-regulated transcripts, we performed ANOVA (FDR-corrected p<0.05) and required the fold change to exceed 1.5.
 
Overall design Single-end RNA sequencing of total RNA extracted from colon crypts obtained from Stat5 knockout, wildtype, and overexpressing mice.
 
Contributor(s) Karns R, Han X
Citation(s) 30948494
Submission date Apr 02, 2018
Last update date Apr 09, 2019
Contact name Rebekah Karns
E-mail Rebekah.Karns@cchmc.org
Organization name Cincinnati Children's Hospital Medical Center
Department Gastroenterology, Hepatology, & Nutrition
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45276
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (10)
GSM3073919 HX1_Stat5 control 1
GSM3073920 HX2_Stat5 KO 1
GSM3073921 HX3_Stat5 OE 1
Relations
BioProject PRJNA448447
SRA SRP136954

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112607_RAW.tar 4.1 Mb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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