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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 01, 2008 |
| Title |
Role of epithelial to mesenchymal transition (EMT) in spontaneous breast cancer metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Epithelial-mesenchymal transition (EMT) has been linked to cancer progression and metastatic propensity. The 4T1 tumor is a clinically relevant model of spontaneous breast cancer metastasis. Here we characterize 4T1-derived cell lines for EMT, in vitro invasiveness and in vivo metastatic ability. Contrary to expectations, the 67NR cells, which form primary tumors but fail to metastasize, express vimentin and N-cadherin, but not E-cadherin. 4T1 cells, however, express E-cadherin, are highly migratory and invasive, and metastasize to multiple sites. The 66cl4 metastatic cells display mixed epithelial and mesenchymal markers, but are less migratory and invasive than 67NR cells. These findings demonstrate that the metastatic ability of breast cancer cells does not correlate with genotypic and phenotypic properties of EMT per se, and suggest that other processes may govern metastatic capability. Gene expression analysis also has not identified differences in EMT markers, but has identified several candidate genes that may influence metastatic ability.
Keywords: cell type comparison
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| Overall design |
Female BALB/c mice were injected with 1x10^6 viable cells (3 mice with 4T1/CMVLUC, 3 mice with 66cl4/CMVLUC, and 3 mice with 67NR/CMVLUC) into the right fourth mammary gland. 15 days after injection primary tumors were excised, and total RNA for microarray hybridization was isolated from the tumor part of laser capture microdissected sections.
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| Contributor(s) |
Lou Y, Preobrazhenska O, auf dem Keller U, Sutcliffe M, Barclay L, McDonald PC, Roskelley C, Overall CM, Dedhar S |
| Citation(s) |
18773493 |
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| Submission date |
Apr 24, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Shoukat Dedhar |
| Organization name |
BC Cancer Research Centre
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| Street address |
675 West 10th Avenue
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| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA106771 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11259_RAW.tar |
32.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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