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Series GSE11228 Query DataSets for GSE11228
Status Public on Jul 25, 2008
Title Human dermal lymphatic endothelial cells stimulated with VEGF-A or VEGF-C for 1h, 4h, 8h and 24h
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Lymphatic vessel growth and activation, mediated by vascular endothelial growth factor- (VEGF)-C and/or VEGF-A, play an important role in metastatic cancer spread and in chronic inflammation. We aimed to comprehensively identify downstream molecular targets induced by VEGF-A or VEGF-C in lymphatic endothelium. To this end, we treated human dermal lymphatic endothelial cells (LEC) with VEGF-A or VEGF-C for up to 24 hours, followed by a time-series transcriptional profiling using gene microarray technology. We identified a number of genes - many of them not previously known to be involved in lymphangiogenesis - that clustered either as early response genes, transiently induced genes or progressively induced genes. Endothelial specific molecule-1 (ESM-1) was one of the genes that were most potently induced by both VEGF-A and VEGF-C.
Keywords: Time course, growth factor comparison
 
Overall design Overnight serum starved human primary dermal microvascular lymphatic endothelial cells (LEC; passage 5-7) were stimulated with either VEGF-A or VEGF-C for 24 hours, 8 hours, 4 hours and 1 hour. Total RNA from all samples were isolated using TriZol at time point zero (control reference point). Treatments were performed at three independent times (triplicates) for each growth factor and at each time point (except the control reference time point), total of 27 samples and labeled and hybridized using the AB1700 microarray platform.
 
Contributor(s) Shin J, Huggenberger R, Detmar M
Citation(s) 18614759
Submission date Apr 21, 2008
Last update date Jan 27, 2016
Contact name Jay Woo Shin
E-mail jay.shin@gsc.riken.jp
Organization name ETH Zurich
Department Institute of Pharmaceutical Sciences
Lab Michael Detmar
Street address Wolfgang-Paulistrasse 10; HCI H394
City Zurich
State/province Zurich
ZIP/Postal code 8093
Country Switzerland
 
Platforms (1)
GPL2986 ABI Human Genome Survey Microarray Version 2
Samples (27)
GSM283080 LEC 0h control - trial 1
GSM283081 LEC 0h control - trial 2
GSM283082 LEC 0h control - trial 3
Relations
BioProject PRJNA106721

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11228_RAW.tar 7.8 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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