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Series GSE1121 Query DataSets for GSE1121
Status Public on Mar 10, 2004
Title changes of global gene expression in E. coli during an oxygen shift
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary The purpose of this study is to investigate the changes of global gene expression in E. coli during an oxygen shift. All cultures were grown under aerobic or anaerobic conditions in M9 minimal media supplemented with glucose. Samples were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent, and total RNA was isolated from exponentially growing cells using a Qiagen RNeasy mini kit (protocols available at www1.qiagen.com). The RNA (10 µg) was then used as the template for cDNA synthesis, the product of which was fragmented, labelled, and hybridized to an Affymetrix E. coli Antisense Genome Array, which was washed and scanned to obtain an image. All of these steps were performed according to Affymetrix protocols (available at www.affymetrix.com).

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Contributor(s) Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO
Citation(s) 15129285
Submission date Mar 09, 2004
Last update date Dec 28, 2012
Contact name Jinseong Cheong
E-mail(s) jcheong@ucsd.edu
Phone (858)534-8758
Organization name University of California at San Diego
Street address
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (43)
GSM18235 aerobic_appY_rep1
GSM18236 aerobic_appY_rep2
GSM18237 aerobic_appY_rep3
This SuperSeries is composed of the following SubSeries:
GSE1106 aerobic knock-out
GSE1107 anaerobic knock-out
Relations
BioProject PRJNA87233

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE1121_RAW.tar 91.6 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file

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