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Series GSE112049 Query DataSets for GSE112049
Status Public on Nov 01, 2018
Title The transcription factor Myb enhances CD8+ T cell stemness and antitumor immunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Stem cells are maintained by transcriptional programs promoting self-renewal and repressing differentiation. Here, we show that the transcription factor Myb is essential for generating and maintaining stem cells within the CD8+ T-cell memory compartment. We found that, following viral infection, CD8+ T cells lacking Myb underwent terminal differentiation and exhibited impaired capacity to form CD62L+ stem cell-like memory cells. Myb acted both as a transcriptional activator of Tcf7 to enhance the development of memory cells and as a repressor of effector differentiation through Zeb2 suppression. Myb mutants’ complementation revealed that the Myb transactivation domain was necessary to restrain terminal differentiation, whereas the negative regulatory domain was critical for cell survival. Conversely, Myb overexpression enhanced CD8+ T-cell memory formation, polyfunctionality, and recall responses promoting curative antitumor immunity. These findings identify Myb as a pivotal regulator of CD8+ T-cell stemness and highlight the therapeutic potential of increasing Myb activity in CD8+ T cells.
Overall design We compared the gene expression profiles of Myb-defiecient CD8+ T cells with those of Myb-sufficient CD8+ T cells
Contributor(s) Gattinoni L, Ji Y
Citation(s) 30778251
Submission date Mar 19, 2018
Last update date Jan 25, 2021
Contact name Luca Gattinoni
Organization name National Cancer Institute
Department Center for Cancer Research
Lab Experimental Transplantation and Immunology Branch
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (6)
GSM3053836 KO1: Myb-deficient CD8+ T cells replicate 1
GSM3053837 KO2: Myb-deficient CD8+ T cells replicate 2
GSM3053838 KO3: Myb-deficient CD8+ T cells replicate 3
BioProject PRJNA439010
SRA SRP136031

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Supplementary file Size Download File type/resource
GSE112049_rnaseq_matrix.txt.gz 751.1 Kb (ftp)(http) TXT
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