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Series GSE11200 Query DataSets for GSE11200
Status Public on Mar 05, 2009
Title Expression data from malting barley seeds
Organism Hordeum vulgare
Experiment type Expression profiling by array
Summary Malting is seed germination under strictly controlled environmental conditions. Malting quality is a complex phenotype that combines a large number of interrelated components, each of which shows complex inheritance. Currently, only a few genes involved in determining malting quality have been characterized. This study combined transcript profiling with phenotypic correlations to identify candidate genes for malting quality.
We used the Barley1 GeneChip® array to identify differentially expressed genes in four malting stages relative to dry seed in the barley variety Morex, and to identify differentially expressed genes among four barley varieties.
Keywords: time course and genotype differences
Overall design Four malting barley cultivars were micromalted: Morex and Legacy (6-row), Merit and Harrington (2-row). Two to three batches of micromalting (biological replications) were performed. For each micromalting experiment, 20 g samples were collected for Morex at four stages: steeping, 24 h germination (day 1), 93 h germination (day 4), and finished malt after kilning was completed. Ungerminated, dry seed of Morex was used as reference sample. In another experiment, seeds of Legacy, Harrington, and Merit were micromalted and 20 g samples were collected for each variety during day 1 and day 4 germination stages. Expression profiles were compared among the four cultivars separately for day 1 and day 4.
Contributor(s) Lapitan N, Hess A, Botha AM, Cooper B, Close T, Lawrence C, Menert J, Badillo D
Citation(s) 19132335
Submission date Apr 17, 2008
Last update date Mar 19, 2012
Contact name Nora L Lapitan
E-mail(s) Nora.Lapitan@ColoState.EDU
Phone 970 491-1921
Fax 970 491-0564
Organization name Colorado State University
Department Soil and Crop Sciences
Lab Crop Genomics Lab
Street address Plant Science Bldg
City Fort Collins
State/province CO
ZIP/Postal code 80523
Country USA
Platforms (1)
GPL1340 [Barley1] Affymetrix Barley Genome Array
Samples (22)
GSM282020 Malting_day4_Legacy_rep 3
GSM282021 Malting_day4_Legacy_rep 2
GSM282022 Malting_day4_Merit_rep4
BioProject PRJNA106797

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11200_RAW.tar 74.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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