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| Status |
Public on Mar 05, 2009 |
| Title |
Expression data from malting barley seeds |
| Organism |
Hordeum vulgare |
| Experiment type |
Expression profiling by array
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| Summary |
Malting is seed germination under strictly controlled environmental conditions. Malting quality is a complex phenotype that combines a large number of interrelated components, each of which shows complex inheritance. Currently, only a few genes involved in determining malting quality have been characterized. This study combined transcript profiling with phenotypic correlations to identify candidate genes for malting quality.
We used the Barley1 GeneChip® array to identify differentially expressed genes in four malting stages relative to dry seed in the barley variety Morex, and to identify differentially expressed genes among four barley varieties.
Keywords: time course and genotype differences
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| Overall design |
Four malting barley cultivars were micromalted: Morex and Legacy (6-row), Merit and Harrington (2-row). Two to three batches of micromalting (biological replications) were performed. For each micromalting experiment, 20 g samples were collected for Morex at four stages: steeping, 24 h germination (day 1), 93 h germination (day 4), and finished malt after kilning was completed. Ungerminated, dry seed of Morex was used as reference sample. In another experiment, seeds of Legacy, Harrington, and Merit were micromalted and 20 g samples were collected for each variety during day 1 and day 4 germination stages. Expression profiles were compared among the four cultivars separately for day 1 and day 4.
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| Contributor(s) |
Lapitan N, Hess A, Botha AM, Cooper B, Close T, Lawrence C, Menert J, Badillo D |
| Citation(s) |
19132335 |
| Submission date |
Apr 17, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Nora L Lapitan |
| E-mail(s) |
Nora.Lapitan@ColoState.EDU
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| Phone |
970 491-1921
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| Fax |
970 491-0564
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| Organization name |
Colorado State University
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| Department |
Soil and Crop Sciences
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| Lab |
Crop Genomics Lab
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| Street address |
Plant Science Bldg
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| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80523 |
| Country |
USA |
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| Platforms (1) |
| GPL1340 |
[Barley1] Affymetrix Barley Genome Array |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA106797 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11200_RAW.tar |
74.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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