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Series GSE111968 Query DataSets for GSE111968
Status Public on Apr 03, 2018
Title Transcriptomic analysis of human tonsillar TFH subsets
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We used an IL4-capture assay followed by FACS sorting, to isolate IL4-secreting TFH cells from a human tonsil and compared their transcriptomic profiles with CXCR5hi PD1hi IL4-negative tonsillar TFH cells and IL4-producing CXCR5neg non-TFH cells (TH2 cells). Our studies validate the notion of functionally distinct TFH subsets and identify genes that are specifically expressed in and define the human IL-4 secreting TFH cell subset.
 
Overall design T follicular helper cell subset mRNA profiles from human tonsils were generated by deep sequencing. Naive CD4 T cells, IL4-producing nonTFH CD4 T cells (i.e. TH2), and IL4-producing TFH cells and PD1hi TFH cells were analyzed.

DOI: 10.26508/lsa.201800050
 
Contributor(s) Mahajan VS, Mattoo H, Pillai S
Citation(s) 29984361
Maehara, T. et al. 2018. The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo. Life Science Alliance. Volume 1, number 1.
NIH grant(s)
Grant ID Grant title Affiliation Name
K08 AI113163 Pathogenesis of IgG4 related disease BRIGHAM AND WOMEN'S HOSPITAL Vinay Subhash Mahajan
U19 AI110495 An Autoimmune center of excellence for the study of IgG4-related disease MASSACHUSETTS GENERAL HOSPITAL SHIV Subramaniam PILLAI
Submission date Mar 16, 2018
Last update date May 21, 2019
Contact name Vinay Mahajan
Organization name Ragon Institute of MGH, MIT and Harvard
Street address 400 Technology Square
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM3045783 Naive_CD4_rep_1
GSM3045784 Naive_CD4_rep_2
GSM3045785 IL4pos_nonTFH_rep_1
Relations
BioProject PRJNA438695
SRA SRP135900

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111968_RAW.tar 32.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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