NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE111747 Query DataSets for GSE111747
Status Public on Mar 14, 2018
Title Different neuronal activity patterns induce different gene expression programs [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A vast number of different neuronal activity patterns could each induce a different set of activity-regulated genes. Mapping this coupling between activity pattern and gene induction would allow inference of a neuron's activity-pattern history from its gene expression and deepen our mechanistic understanding of activity pattern-dependent synaptic plasticity. In genome-scale experiments comparing brief and sustained activity patterns, we reveal that activity-pattern history can indeed be inferred from gene expression state. We find that brief activity selectively induces a small subset of the activity-regulated gene program that corresponds to the first of three temporal waves of genes induced by sustained activity. These first-wave genes are mechanistically distinct from genes in the later waves because their induction requires MAPK/ERK signaling but does not require de novo translation. Thus, the same mechanisms that establish the multi-wave temporal structure of gene induction also enable different gene sets to be induced by different activity patterns.
 
Overall design Gene expression profiles of neurons in the presence/absense of MEK inhibition and neuronal activity.
 
Contributor(s) Tyssowski KM
Citation(s) 29681534
Submission date Mar 13, 2018
Last update date Mar 25, 2019
Contact name Kelsey Tyssowski
E-mail(s) tyssowski@fas.harvard.edu
Organization name Harvard Medical School
Department Genetics
Lab Gray (gray@genetics.med.harvard.edu)
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02120
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (186)
GSM3038993 sample1: unstim rep 1.1, veh, total RNA
GSM3038994 sample2: unstim rep 2.1, veh, total RNA
GSM3038995 sample3: unstim rep 1.2, veh, total RNA
This SubSeries is part of SuperSeries:
GSE111899 Different neuronal activity patterns induce different gene expression programs
Relations
BioProject PRJNA438061
SRA SRP135246

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111747_Table_S2.xlsx 9.1 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap