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Series GSE111588 Query DataSets for GSE111588
Status Public on Sep 01, 2018
Title Single cell RNA-sequencing of visceral adipose tissue Pdgfrβ+ cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We previously derived a doxycycline-inducible (Tet-On) lineage-tracing model that allows for the indelible labeling of Pdgfrb-expressing perivascular cells in adipose tissue of adult mice (PdgfrbrtTA; TRE-Cre; Rosa26RmT/mG; herein, “MuralChaser mice”). Prior to exposing animals to doxycyline, all cells within the stromal-vascular fraction (SVF) of adult gonadal WAT (gWAT) express membrane tdTomato from the Rosa26 locus. Following 9 days of exposure to doxycycline-containing chow diet, Cre-mediated excision of the loxP-flanked tdTomato cassette occurs in Pdgfrb-expressing cells, and membrane-bound GFP (mGFP) expression is constitutively activated. We set out to test the hypothesis that Pdgfrb-expressing perivascular cells in gonadal visceral WAT of adult mice are heterogeneous, with subpopulations harboring functionally distinct phenotypes. To this end, we performed single cell RNA-sequencing of mGFP+ cells isolated from gWAT of lean (chow fed) 8 weeks-old male MuralChaser mice following 9 days of doxycycline exposure.
Overall design We pooled tdTomato- GFP+ cells from gonadal white adipose tissue (WAT) from 5 MuralChaser mice. Single cell library preparation was performed using the 10X Genomics Single Cell 3’ v2 according to the manufacturer’s instructions.
Contributor(s) Gupta RK, Hepler C
Citation(s) 30265241
Submission date Mar 08, 2018
Last update date Jan 27, 2020
Contact name Rana K Gupta
Organization name UT Southwestern Medical Center
Department Touchstone Diabetes Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province Texas
ZIP/Postal code 75287
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM3034641 Gonadal tdTomato- GFP+ cells from gonadal WAT
This SubSeries is part of SuperSeries:
GSE144299 Single cell RNA-sequencing of gonadal and inguinal adipose tissue Pdgfrβ+ cells
BioProject PRJNA437472
SRA SRP134197

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Supplementary file Size Download File type/resource
GSE111588_RAW.tar 12.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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