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Series GSE11128 Query DataSets for GSE11128
Status Public on Aug 22, 2008
Title Expression data from single cells from mouse primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative-PCR analysis, we defined the genome-wide transcription dynamics that create primordial germ cells (PGCs) from the epiblast, a process that exclusively segregates them from their somatic neighbors. We also analyzed the effect of the loss of Blimp1, a key transcriptional regulator, on these dynamics. Our analysis revealed that PGC specification involves complex, yet highly ordered regulation of a large number of genes, proceeding under the strong influence of mesoderm induction with active repression of specific programs such as epithelial-mesenchymal transition, Hox gene activation, cell-cycle progression and DNA methyltransferase machinery. Remarkably, Blimp1 is essential for repressing nearly all the genes normally down-regulated in PGCs relative to their somatic neighbors, whereas it is dispensable for the activation of approximately half of the genes up-regulated in PGCs.
Keywords: single cell analysis, time course, Blimp1 knocked out
Overall design Embryo isolation, dissection, and single cell cDNA amplification were performed as described (Kurimot et al., 2006, Nucleic Acids Res 34: e42; Kurimoto et al., 2007, Nature protocols, 2007, 2:739-52). Randomly picked cells were screened with gene-specific primers for Blimp1 and fragilis to select Blimp1-positive embryonic cells (which marks lineage-restricted PGC precursors or PGCs). For Blimp1 KO embryos, non-functional transcript from the Blimp1null allele was detected with the same primer set.
Contributor(s) Kurimoto K, Yabuta Y, Ohinata Y, Shigeta M, Yamanaka K, Saitou M
Citation(s) 18559478
Submission date Apr 10, 2008
Last update date Feb 11, 2019
Contact name Kazuki Kurimoto
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (106)
GSM280665 single cell from posterior epiblast(Pre-streak), biological rep1
GSM280666 single cell from posterior epiblast(Pre-streak), biological rep2
GSM280667 single cell from posterior epiblast(Pre-streak), biological rep3
BioProject PRJNA106911

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Supplementary file Size Download File type/resource
GSE11128_RAW.tar 606.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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