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Series GSE11128 Query DataSets for GSE11128
Status Public on Aug 22, 2008
Title Expression data from single cells from mouse primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative-PCR analysis, we defined the genome-wide transcription dynamics that create primordial germ cells (PGCs) from the epiblast, a process that exclusively segregates them from their somatic neighbors. We also analyzed the effect of the loss of Blimp1, a key transcriptional regulator, on these dynamics. Our analysis revealed that PGC specification involves complex, yet highly ordered regulation of a large number of genes, proceeding under the strong influence of mesoderm induction with active repression of specific programs such as epithelial-mesenchymal transition, Hox gene activation, cell-cycle progression and DNA methyltransferase machinery. Remarkably, Blimp1 is essential for repressing nearly all the genes normally down-regulated in PGCs relative to their somatic neighbors, whereas it is dispensable for the activation of approximately half of the genes up-regulated in PGCs.
Keywords: single cell analysis, time course, Blimp1 knocked out
 
Overall design Embryo isolation, dissection, and single cell cDNA amplification were performed as described (Kurimot et al., 2006, Nucleic Acids Res 34: e42; Kurimoto et al., 2007, Nature protocols, 2007, 2:739-52). Randomly picked cells were screened with gene-specific primers for Blimp1 and fragilis to select Blimp1-positive embryonic cells (which marks lineage-restricted PGC precursors or PGCs). For Blimp1 KO embryos, non-functional transcript from the Blimp1null allele was detected with the same primer set.
 
Contributor(s) Kurimoto K, Yabuta Y, Ohinata Y, Shigeta M, Yamanaka K, Saitou M
Citation(s) 18559478
Submission date Apr 10, 2008
Last update date Feb 11, 2019
Contact name Kazuki Kurimoto
E-mail kurimoto@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (106)
GSM280665 single cell from posterior epiblast(Pre-streak), biological rep1
GSM280666 single cell from posterior epiblast(Pre-streak), biological rep2
GSM280667 single cell from posterior epiblast(Pre-streak), biological rep3
Relations
BioProject PRJNA106911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11128_RAW.tar 606.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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